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PMID: 2546040 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning and DNA sequence of plasmid determinant iss, coding for increased serum survival and surface exclusion, which has homology with lambda DNA.

Molecular & general genetics : MGG ·Vol. 216 ·No. 2-3 ·1989-04-00 ·Pages 287-92

Chuba PJ, Leon MA, Banerjee A, Palchaudhuri S

Abstract

Escherichia coli K12 cells carrying a cloned 1.4 kb HindIII fragment from plasmid ColV2-K94, showed increased survival in guinea pig serum. The recombinant plasmid also conferred group II surface exclusion, i.e. the cells were reduced in recipient ability towards the incoming plasmid R538drd in conjugation experiments. Southern blotting suggested homology with bacteriophage lambda DNA and to the insertion element IS2. Determination of the DNA sequence of the fragment demonstrated the presence of a truncated IS2 (165 bp), separated by 250 bp from a 900 bp stretch of homology with lambda DNA, beginning within the Rz gene and continuing in the rightward direction on the lambda map. A 97 amino acid open reading frame (ORF) adjacent to Rz and on the opposite strand, remained intact in iss, with several amino acid changes. The ORF in iss is preceded by sequences resembling prokaryotic ribosome binding sites and promoters.

MeSH Terms
Amino Acid Sequence Bacteriophage lambda/genetics Base Sequence Chromosome Mapping Cloning, Molecular DNA Transposable Elements DNA, Bacterial/genetics DNA, Viral/genetics Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Plasmids Sequence Homology, Nucleic Acid
Chemicals
DNA Transposable Elements DNA, Bacterial DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chuba P J
Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201.
Leon M A
Banerjee A
Palchaudhuri S
References (29)
29 references, click to expand
  1. Physical characterisation of the "Rac prophage" in E. coli K12.
    Mol Gen Genet. 1979 Sep;175(2):159-74 PMID: 390313
  2. Incompatibility repressor in a RepA-like replicon of the IncFI plasmid ColV2-K94.
    J Bacteriol. 1986 Jun;166(3):1106-12 PMID: 2423502
  3. A physical and genetic map of the IncFI plasmid ColV2-K94.
    Plasmid. 1985 May;13(3):215-8 PMID: 2988000
  4. Isolation of high-frequency recombining strains from Escherichia coli containing the V colicinogenic factor.
    J Bacteriol. 1968 Jul;96(1):205-14 PMID: 4874307
  5. Lysis gene t of T-even bacteriophages: evidence that colicins and bacteriophage genes have common ancestors.
    J Bacteriol. 1987 Jul;169(7):2956-61 PMID: 3597316
  6. Plasmid-determined resistance to serum bactericidal activity: a major outer membrane protein, the traT gene product, is responsible for plasmid-specified serum resistance in Escherichia coli.
    Infect Immun. 1980 May;28(2):359-67 PMID: 6995306
  7. Nucleotide sequence homologies in control regions of prokaryotic genomes.
    Gene. 1987;58(1):45-57 PMID: 2826299
  8. The conjugation system of F-like plasmids.
    Annu Rev Genet. 1980;14:41-76 PMID: 6111288
  9. Nucleotide sequence of the transposable DNA-element IS2.
    Nucleic Acids Res. 1979 Mar;6(3):1111-22 PMID: 375194
  10. Effect of lysogeny on serum sensitivity.
    J Bacteriol. 1968 Dec;96(6):1912-4 PMID: 4881698
  11. Further characterization of complement resistance conferred on Escherichia coli by the plasmid genes traT of R100 and iss of ColV,I-K94.
    Infect Immun. 1982 Feb;35(2):654-9 PMID: 7035371
  12. A genetic analysis of F sex factor cistrons needed for surface exclusion in Escherichia coli.
    J Mol Biol. 1980 Apr 25;138(4):779-95 PMID: 6997497
  13. The association of the O18, K1 and H7 antigens and the Co1V plasmid of a strain of Escherichia coli with its virulence and immunogenicity.
    J Gen Microbiol. 1980 Dec;121(2):387-400 PMID: 7021764
  14. Genetic structure and stability of a copy number mutant of IncFI group plasmid ColV-K94 in Escherichia coli K-12.
    Mol Gen Genet. 1984;193(2):349-57 PMID: 6319972
  15. Unexpected relationships between bacteriophage lambda hypothetical proteins and bacteriophage T4 tail-fiber proteins.
    Biochem Biophys Res Commun. 1983 Sep 30;115(3):1061-8 PMID: 6226290
  16. Identification of a second cryptic lambdoid prophage locus in the E. coli K12 chromosome.
    Mol Gen Genet. 1980;180(2):479-81 PMID: 6258029
  17. Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K-12.
    Mol Gen Genet. 1973 May 9;122(3):267-77 PMID: 4577900
  18. Sequence rearrangements in the plasmid ColV,I-K94.
    Plasmid. 1982 Jul;8(1):55-72 PMID: 6292970
  19. Cloned fragments of the plasmid ColV,I-K94 specifying virulence and serum resistance.
    Nature. 1979 Jun 28;279(5716):778-81 PMID: 377103
  20. Nucleotide sequence of bacteriophage lambda DNA.
    J Mol Biol. 1982 Dec 25;162(4):729-73 PMID: 6221115
  21. Relative contribution of ColV plasmid and K1 antigen to the pathogenicity of Escherichia coli.
    Infect Immun. 1983 Apr;40(1):359-68 PMID: 6339405
  22. Genetic organization of insertion element IS2 based on a revised nucleotide sequence.
    Gene. 1987;59(2-3):291-6 PMID: 2830172
  23. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  24. Second replicon in ColV2-K94 mediates the stable coexistence of two incompatible plasmids.
    J Bacteriol. 1984 Oct;160(1):245-50 PMID: 6090416
  25. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  26. Characterization of the terminal sequences flanking the transposon that carries the Escherichia coli enterotoxin STII gene.
    Gene. 1987;55(2-3):157-67 PMID: 2822540
  27. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors.
    J Mol Biol. 1973 Apr 5;75(2):235-55 PMID: 4580676
  28. Nucleotide sequence analysis of the complement resistance gene from plasmid R100.
    J Bacteriol. 1982 Aug;151(2):819-27 PMID: 6284713
  29. Physical mapping of a DNA sequence common to plasmids of incompatibility group F I.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1190-4 PMID: 322149
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334367
Published
1989-04-00
Pages
287-92
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIAID NIH HHS · AI21273 · United States
External Links
PubMed source DOI: 10.1007/BF00334367
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