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PMID: 2349239 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The ability of Salmonella to enter mammalian cells is affected by bacterial growth state.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 11 ·1990-06-00 ·Pages 4304-8

Lee CA, Falkow S

Abstract

We have examined the effect of different growth conditions on the ability of Salmonella to interact with Madin-Darby canine kidney cells. Two growth conditions that affect the expression of Salmonella adherence and invasiveness have been identified. First, bacteria lose their invasiveness in the stationary phase of growth. Second, bacteria growing in oxygen-limited growth conditions are induced for adherence and invasiveness, whereas those growing aerobically are relatively nonadherent and noninvasive. Salmonella from cultures aerated with gas mixtures containing 0% or 1% oxygen were 6- to 70-fold more adherent and invasive than those from cultures aerated with a gas mixture containing 20% oxygen. The Salmonella typhimurium oxrA gene that is required for the anaerobic induction of many proteins is not involved in the regulation of Salmonella invasiveness. We speculate that oxygen limitation might be an environmental cue that triggers the expression of Salmonella invasiveness within the intestinal lumen and other tissues.

MeSH Terms
Anaerobiosis Animals Bacterial Adhesion Bacterial Proteins/biosynthesis Cells, Cultured/microbiology Dogs Kidney/cytology Molecular Weight Oxygen/physiology Salmonella/growth & development,pathogenicity
Chemicals
Bacterial Proteins Oxygen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lee C A
Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305-5402.
Falkow S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.11.4304
Published
1990-06-00
Pages
4304-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54097
Subset
IM
Grants
NIAID NIH HHS · AI26195 · United States
NIDDK NIH HHS · DK38707 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.11.4304 PMC Full Text View journal details
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