Home LiteratureArticle Details
PMID: 8195290 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interactions of tensin with actin and identification of its three distinct actin-binding domains.

The Journal of cell biology ·Vol. 125 ·No. 5 ·1994-06-00 ·Pages 1067-75

Lo SH, Janmey PA, Hartwig JH, Chen LB

Abstract

Tensin, a 200-kD phosphoprotein of focal contacts, contains sequence homologies to Src (SH2 domain), and several actin-binding proteins. These features suggest that tensin may link the cell membrane to the cytoskeleton and respond directly to tyrosine kinase signalling pathways. Here we identify three distinct actin-binding domains within tensin. Recombinant tensin purified after overexpression by a baculovirus system binds to actin filaments with Kd = 0.1 microM, cross-links actin filaments at a molar ratio of 1:10 (tensin/actin), and retards actin assembly by barbed end capping with Kd = 20 nM. Tensin fragments were constructed and expressed as fusion proteins to map domains having these activities. Three regions from tensin interact with actin: two regions composed of amino acids 1 to 263 and 263 to 463, cosediment with F-actin but do not alter the kinetics of actin assembly; a region composed of amino acids 888-989, with sequence homology to insertin, retards actin polymerization. A claw-shaped tensin dimer would have six potential actin-binding sites and could embrace the ends of two actin filaments at focal contacts.

MeSH Terms
Actin Cytoskeleton/ultrastructure Actins/metabolism,ultrastructure Animals Binding Sites Chickens Microfilament Proteins/metabolism Muscle Contraction Protein Binding Recombinant Fusion Proteins Tensins
Chemicals
Actins Microfilament Proteins Recombinant Fusion Proteins Tensins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lo S H
Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Janmey P A
Hartwig J H
Chen L B
References (54)
54 references, click to expand
  1. Coupled expression and colocalization of 140K cell adhesion molecules, fibronectin, and laminin during morphogenesis and cytodifferentiation of chick lung cells.
    J Cell Biol. 1986 Sep;103(3):1073-90 PMID: 3528168
  2. Focal adhesion as a signal transduction organelle.
    Cancer Metastasis Rev. 1994 Mar;13(1):9-24 PMID: 8143347
  3. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.
    J Biol Chem. 1971 Aug 10;246(15):4866-71 PMID: 4254541
  4. Alpha-actinin: immunofluorescent localization of a muscle structural protein in nonmuscle cells.
    Cell. 1975 Nov;6(3):289-98 PMID: 802682
  5. Practical aspects of immune electron microscopy.
    Methods Enzymol. 1979;61:250-7 PMID: 384141
  6. A 130K protein from chicken gizzard: its localization at the termini of microfilament bundles in cultured chicken cells.
    Cell. 1979 Sep;18(1):193-205 PMID: 574428
  7. A rapid purification of alpha-actinin, filamin, and a 130,000-dalton protein from smooth muscle.
    J Biol Chem. 1980 Feb 10;255(3):1194-9 PMID: 7356657
  8. Adhesion plaques of Rous sarcoma virus-transformed cells contain the src gene product.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3514-8 PMID: 6251464
  9. Fluorimetry study of N-(1-pyrenyl)iodoacetamide-labelled F-actin. Local structural change of actin protomer both on polymerization and on binding of heavy meromyosin.
    Eur J Biochem. 1981;114(1):33-8 PMID: 7011802
  10. A new protein of adhesion plaques and ruffling membranes.
    J Cell Biol. 1983 Aug;97(2):359-67 PMID: 6684120
  11. Expression of transformation-associated protease(s) that degrade fibronectin at cell contact sites.
    J Cell Biol. 1984 Apr;98(4):1546-55 PMID: 6325472
  12. An interaction between vinculin and talin.
    Nature. 1984 Apr 19-25;308(5961):744-6 PMID: 6425696
  13. Synapsin I bundles F-actin in a phosphorylation-dependent manner.
    Nature. 1987 Apr 16-22;326(6114):704-7 PMID: 3104800
  14. Colocalization of calcium-dependent protease II and one of its substrates at sites of cell adhesion.
    Cell. 1987 Nov 20;51(4):569-77 PMID: 2824061
  15. Cellular transformation, tyrosine kinase oncogenes, and the cellular adhesion plaque.
    Bioessays. 1988 Jan;8(1):25-30 PMID: 3130051
  16. Focal adhesions and cell-matrix interactions.
    Coll Relat Res. 1988 Mar;8(2):155-82 PMID: 3288433
  17. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton.
    Annu Rev Cell Biol. 1988;4:487-525 PMID: 3058164
  18. Kinetic evidence for insertion of actin monomers between the barbed ends of actin filaments and barbed end-bound insertin, a protein purified from smooth muscle.
    J Mol Biol. 1989 Nov 5;210(1):141-8 PMID: 2555521
  19. A new 400-kD protein from isolated adherens junctions: its localization at the undercoat of adherens junctions and at microfilament bundles such as stress fibers and circumferential bundles.
    J Cell Biol. 1989 Dec;109(6 Pt 1):2905-15 PMID: 2687289
  20. Identification of a filamin isoform enriched at the ends of stress fibers in chicken embryo fibroblasts.
    J Cell Sci. 1989 Sep;94 ( Pt 1):109-18 PMID: 2693470
  21. Cytoskeleton-associated cell contacts.
    Curr Opin Cell Biol. 1989 Feb;1(1):103-9 PMID: 2698202
  22. Mitosis-specific phosphorylation causes 83K non-muscle caldesmon to dissociate from microfilaments.
    Nature. 1990 Apr 12;344(6267):675-8 PMID: 2157986
  23. Identification of a short sequence essential for actin binding by Dictyostelium ABP-120.
    J Biol Chem. 1990 Jun 5;265(16):9236-40 PMID: 2345173
  24. An interaction between alpha-actinin and the beta 1 integrin subunit in vitro.
    J Cell Biol. 1990 Aug;111(2):721-9 PMID: 2116421
  25. Paxillin: a new vinculin-binding protein present in focal adhesions.
    J Cell Biol. 1990 Sep;111(3):1059-68 PMID: 2118142
  26. Atomic structure of the actin:DNase I complex.
    Nature. 1990 Sep 6;347(6288):37-44 PMID: 2395459
  27. Atomic model of the actin filament.
    Nature. 1990 Sep 6;347(6288):44-9 PMID: 2395461
  28. Direct interactions between talin and actin.
    Biochem Biophys Res Commun. 1990 Sep 28;171(3):1217-23 PMID: 2121138
  29. Molecular structure of F-actin and location of surface binding sites.
    Nature. 1990 Nov 15;348(6298):217-21 PMID: 2234090
  30. Purification and characterization of zyxin, an 82,000-dalton component of adherens junctions.
    J Biol Chem. 1991 Mar 25;266(9):5847-53 PMID: 2005121
  31. Radixin, a barbed end-capping actin-modulating protein, is concentrated at the cleavage furrow during cytokinesis.
    J Cell Biol. 1991 Apr;113(2):321-30 PMID: 1707055
  32. SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins.
    Science. 1991 May 3;252(5006):668-74 PMID: 1708916
  33. Presence of an SH2 domain in the actin-binding protein tensin.
    Science. 1991 May 3;252(5006):712-5 PMID: 1708917
  34. The 102 kd cadherin-associated protein: similarity to vinculin and posttranscriptional regulation of expression.
    Cell. 1991 May 31;65(5):849-57 PMID: 1904011
  35. Mechanism of the insertion of actin monomers between the barbed ends of actin filaments and barbed end-bound insertin.
    J Muscle Res Cell Motil. 1991 Feb;12(1):27-36 PMID: 2050808
  36. Reconstitution of functional receptors for human granulocyte/macrophage colony-stimulating factor (GM-CSF): evidence that the protein encoded by the AIC2B cDNA is a subunit of the murine GM-CSF receptor.
    Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5082-6 PMID: 1828890
  37. Modular organization of actin crosslinking proteins.
    Trends Biochem Sci. 1991 Mar;16(3):87-92 PMID: 2058002
  38. Evidence that a 27-residue sequence is the actin-binding site of ABP-120.
    J Biol Chem. 1991 Jul 15;266(20):12989-93 PMID: 2071586
  39. Actin-binding proteins.
    Curr Opin Cell Biol. 1991 Feb;3(1):87-97 PMID: 1854489
  40. Transmembrane molecular assemblies in cell-extracellular matrix interactions.
    Curr Opin Cell Biol. 1991 Oct;3(5):849-53 PMID: 1931085
  41. Comparison of protein tyrosine phosphorylation and morphological changes induced by IL-2 and IL-3.
    Int Immunol. 1991 Nov;3(11):1137-48 PMID: 1760408
  42. MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin.
    Nature. 1992 Apr 16;356(6370):618-22 PMID: 1560845
  43. Effect of villin on the kinetics of actin polymerization.
    Biochemistry. 1984 Jun 5;23(12):2613-21 PMID: 6432033
  44. Purification and characterization of a protein from chicken gizzard, which inhibits actin polymerization.
    Eur J Biochem. 1985 Dec 16;153(3):515-20 PMID: 4076188
  45. A re-examination of the interaction of vinculin with actin.
    J Cell Biol. 1986 Mar;102(3):1085-92 PMID: 3005334
  46. Interaction of plasma membrane fibronectin receptor with talin--a transmembrane linkage.
    Nature. 1986 Apr 10-16;320(6062):531-3 PMID: 2938015
  47. Structure and mobility of actin filaments as measured by quasielastic light scattering, viscometry, and electron microscopy.
    J Biol Chem. 1986 Jun 25;261(18):8357-62 PMID: 3013849
  48. pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions.
    Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5192-6 PMID: 1594631
  49. The 46/50 kDa phosphoprotein VASP purified from human platelets is a novel protein associated with actin filaments and focal contacts.
    EMBO J. 1992 Jun;11(6):2063-70 PMID: 1318192
  50. Occurrence of an actin-inserting domain in tensin.
    J Mol Biol. 1992 Sep 20;227(2):593-5 PMID: 1404377
  51. SH2 and SH3 domains: from structure to function.
    Cell. 1992 Oct 30;71(3):359-62 PMID: 1423600
  52. Cytoskeleton--plasma membrane interactions.
    Science. 1992 Nov 6;258(5084):955-64 PMID: 1439807
  53. Autonomous expression of a noncatalytic domain of the focal adhesion-associated protein tyrosine kinase pp125FAK.
    Mol Cell Biol. 1993 Feb;13(2):785-91 PMID: 8423801
  54. Rate constants and equilibrium constants for binding of the gelsolin-actin complex to the barbed ends of actin filaments in the presence and absence of calcium.
    Eur J Biochem. 1986 Oct 15;160(2):379-87 PMID: 3021456
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.125.5.1067
Published
1994-06-00
Pages
1067-75
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120063
Subset
IM
Grants
NIDDK NIH HHS · DK 38452 · United States
NIGMS NIH HHS · GM 38318 · United States
NHLBI NIH HHS · HL 47874 · United States
External Links
PubMed source DOI: 10.1083/jcb.125.5.1067 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2