Home LiteratureArticle Details
PMID: 8423801 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Autonomous expression of a noncatalytic domain of the focal adhesion-associated protein tyrosine kinase pp125FAK.

Molecular and cellular biology ·Vol. 13 ·No. 2 ·1993-02-00 ·Pages 785-91

Schaller MD, Borgman CA, Parsons JT

Abstract

Integrins play a central role in cellular adhesion and anchorage of the cytoskeleton and participate in the generation of intracellular signals, including tyrosine phosphorylation. We have recently isolated a cDNA encoding a unique, focal adhesion-associated protein tyrosine kinase (FAK) that is a component of an integrin-mediated signal transduction pathway. Here we report the isolation of cDNAs encoding the C-terminal, noncatalytic domain of the FAK kinase, termed FRNK (FAK-related nonkinase). Both the FAK- and FRNK-encoded polypeptides, pp125FAK and p41/p43FRNK, are expressed in normal chicken embryo cells. pp125FAK and p41/p43FRNK were localized to focal adhesions, suggesting that pp125FAK is directed to the focal adhesions by sequences within its C-terminal domain. We also show that the fibronectin-dependent increase in tyrosine phosphorylation of pp125FAK is accompanied by a concomitant posttranslational modification of p41FRNK.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Blotting, Western Cell Adhesion Cell Adhesion Molecules/genetics,metabolism Chick Embryo DNA Focal Adhesion Kinase 1 Focal Adhesion Protein-Tyrosine Kinases Molecular Sequence Data Phosphorylation Protein Processing, Post-Translational Protein-Tyrosine Kinases/genetics,metabolism
Chemicals
Cell Adhesion Molecules DNA Protein-Tyrosine Kinases Focal Adhesion Kinase 1 Focal Adhesion Protein-Tyrosine Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schaller M D
Department of Microbiology and Cancer Center, University of Virginia Health Sciences Center, Charlottesville 22908.
Borgman C A
Parsons J T
References (35)
35 references, click to expand
  1. Tumor necrosis factor-induced degranulation in adherent human neutrophils is dependent on CD11b/CD18-integrin-triggered oscillations of cytosolic free Ca2+.
    Proc Natl Acad Sci U S A. 1990 Dec;87(23):9472-6 PMID: 1979172
  2. Signal transduction through the fibronectin receptor induces collagenase and stromelysin gene expression.
    J Cell Biol. 1989 Aug;109(2):877-89 PMID: 2547805
  3. Multiple elevations of cytosolic-free Ca2+ in human neutrophils: initiation by adherence receptors of the integrin family.
    J Cell Biol. 1991 Mar;112(6):1249-57 PMID: 1900302
  4. Multiple integrins share the ability to induce elevation of intracellular pH.
    Exp Cell Res. 1991 Aug;195(2):533-5 PMID: 1712734
  5. A novel Ca2+/calmodulin-dependent protein kinase and a male germ cell-specific calmodulin-binding protein are derived from the same gene.
    Mol Cell Biol. 1991 Aug;11(8):3960-71 PMID: 1649385
  6. Consensus sequences as substrate specificity determinants for protein kinases and protein phosphatases.
    J Biol Chem. 1991 Aug 25;266(24):15555-8 PMID: 1651913
  7. Insoluble fibronectin activates the Na/H antiporter by clustering and immobilizing integrin alpha 5 beta 1, independent of cell shape.
    Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7849-53 PMID: 1652767
  8. Identification and characterization of a novel cytoskeleton-associated pp60src substrate.
    Mol Cell Biol. 1991 Oct;11(10):5113-24 PMID: 1922035
  9. Signal transduction by integrins: increased protein tyrosine phosphorylation caused by clustering of beta 1 integrins.
    Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8392-6 PMID: 1717976
  10. Protein tyrosine phosphorylation and the adhesive functions of platelets.
    Curr Opin Cell Biol. 1991 Oct;3(5):869-79 PMID: 1931088
  11. Protein kinase phosphorylation site sequences and consensus specificity motifs: tabulations.
    Methods Enzymol. 1991;200:62-81 PMID: 1956339
  12. Integrins: versatility, modulation, and signaling in cell adhesion.
    Cell. 1992 Apr 3;69(1):11-25 PMID: 1555235
  13. Fibronectin/integrin interaction induces tyrosine phosphorylation of a 120-kDa protein.
    Cell Regul. 1991 Nov;2(11):951-64 PMID: 1725602
  14. pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions.
    Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5192-6 PMID: 1594631
  15. Regulation of focal adhesion-associated protein tyrosine kinase by both cellular adhesion and oncogenic transformation.
    Nature. 1992 Aug 20;358(6388):690-2 PMID: 1379699
  16. Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment to fibronectin.
    Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8487-91 PMID: 1528852
  17. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  18. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
  19. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  20. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  21. Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5794-8 PMID: 6777776
  22. The retinoblastoma protein is phosphorylated during specific phases of the cell cycle.
    Cell. 1989 Sep 22;58(6):1097-105 PMID: 2673543
  23. Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases.
    Proc Natl Acad Sci U S A. 1990 May;87(9):3328-32 PMID: 2110361
  24. Integrins and other cell adhesion molecules.
    FASEB J. 1990 Aug;4(11):2868-80 PMID: 2199285
  25. An interaction between alpha-actinin and the beta 1 integrin subunit in vitro.
    J Cell Biol. 1990 Aug;111(2):721-9 PMID: 2116421
  26. Monoclonal antibodies specific for the carboxy terminus of simian virus 40 large T antigen.
    J Virol. 1984 Nov;52(2):483-91 PMID: 6208378
  27. Interaction of plasma membrane fibronectin receptor with talin--a transmembrane linkage.
    Nature. 1986 Apr 10-16;320(6062):531-3 PMID: 2938015
  28. Adaptor plasmids simplify the insertion of foreign DNA into helper-independent retroviral vectors.
    J Virol. 1987 Oct;61(10):3004-12 PMID: 3041020
  29. Occupation of the extracellular matrix receptor, integrin, is a control point for myogenic differentiation.
    Cell. 1987 Oct 9;51(1):51-7 PMID: 3115595
  30. Tyrosine-specific protein phosphorylation is regulated by glycoprotein IIb-IIIa in platelets.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2234-8 PMID: 2928328
  31. Integrins isolated from Rous sarcoma virus-transformed chicken embryo fibroblasts.
    Oncogene. 1989 Mar;4(3):325-33 PMID: 2468126
  32. Transformation-specific tyrosine phosphorylation of a novel cellular protein in chicken cells expressing oncogenic variants of the avian cellular src gene.
    Mol Cell Biol. 1989 Feb;9(2):629-38 PMID: 2469003
  33. Immunoaffinity purification of tyrosine-phosphorylated cellular proteins.
    J Immunol Methods. 1989 Jun 2;120(1):115-24 PMID: 2471744
  34. Fibronectin inhibits the terminal differentiation of human keratinocytes.
    Nature. 1989 Jul 27;340(6231):307-9 PMID: 2473404
  35. Role of platelet membrane glycoprotein IIb-IIIa in agonist-induced tyrosine phosphorylation of platelet proteins.
    J Cell Biol. 1990 Dec;111(6 Pt 2):3117-27 PMID: 1702789
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.2.785-791.1993
Published
1993-02-00
Pages
785-91
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358961
Subset
IM
Grants
NCI NIH HHS · CA29243 · United States
NCI NIH HHS · CA40042 · United States
Databases
GENBANK
L07415
External Links
PubMed source DOI: 10.1128/mcb.13.2.785-791.1993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2