Home LiteratureArticle Details
PMID: 2116421 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An interaction between alpha-actinin and the beta 1 integrin subunit in vitro.

The Journal of cell biology ·Vol. 111 ·No. 2 ·1990-08-00 ·Pages 721-9

Otey CA, Pavalko FM, Burridge K

Abstract

A number of cytoskeletal-associated proteins that are concentrated in focal contacts, namely alpha-actinin, vinculin, talin, and integrin, have been shown to interact in vitro such that they suggest a potential link between actin filaments and the membrane. Because some of these interactions are of low affinity, we suspect the additional linkages also exist. Therefore, we have used a synthetic peptide corresponding to the cytoplasmic domain of beta 1 integrin and affinity chromatography to identify additional integrin-binding proteins. Here we report our finding of an interaction between the cytoplasmic domain of beta 1 integrin and the actin-binding protein alpha-actinin. Beta 1-integrin cytoplasmic domain peptide columns bound several proteins from Triton extracts of chicken embryo fibroblasts. One protein at approximately 100 kD was identified by immunoblot analysis as alpha-actinin. Solid phase binding assays indicated that alpha-actinin bound specifically and directly to the beta 1 peptide with relatively high affinity. Using purified heterodimeric chicken smooth muscle integrin (a beta 1 integrin) or the platelet integrin glycoprotein IIb/IIIa complex (a beta 3 integrin), binding of alpha-actinin was also observed in similar solid phase assays, albeit with a lower affinity than was seen using the beta 1 peptide. alpha-Actinin also bound specifically to phospholipid vesicles into which glycoprotein IIb/IIIa had been incorporated. These results lead us to suggest that this integrin-alpha-actinin linkage may contribute to the attachment of actin filaments to the membrane in certain locations.

MeSH Terms
Actinin/isolation & purification,metabolism Amino Acid Sequence Animals Cells, Cultured Chick Embryo Chromatography, Affinity Cytoskeletal Proteins/isolation & purification,metabolism Fibroblasts/metabolism Gizzard, Avian/metabolism Immunoblotting Integrins/metabolism Kinetics Macromolecular Substances Molecular Sequence Data Molecular Weight Muscle, Smooth/metabolism Peptides/chemical synthesis Phospholipids/metabolism Vinculin
Chemicals
Cytoskeletal Proteins Integrins Macromolecular Substances Peptides Phospholipids Actinin Vinculin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Otey C A
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599.
Pavalko F M
Burridge K
References (43)
43 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Association of dystrophin and an integral membrane glycoprotein.
    Nature. 1989 Mar 16;338(6212):259-62 PMID: 2493582
  3. Alpha-actinin: immunofluorescent localization of a muscle structural protein in nonmuscle cells.
    Cell. 1975 Nov;6(3):289-98 PMID: 802682
  4. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  5. A rapid purification of alpha-actinin, filamin, and a 130,000-dalton protein from smooth muscle.
    J Biol Chem. 1980 Feb 10;255(3):1194-9 PMID: 7356657
  6. The association of alpha-actinin with the plasma membrane.
    J Supramol Struct. 1980;13(1):53-65 PMID: 7442255
  7. Immunoelectron microscopic studies of the sites of cell-substratum and cell-cell contacts in cultured fibroblasts.
    J Cell Biol. 1982 Oct;95(1):205-22 PMID: 6815205
  8. Detection of vinculin-binding proteins with an 125I-vinculin gel overlay technique.
    J Cell Biol. 1983 Oct;97(4):1283-7 PMID: 6413514
  9. Detection of high molecular weight vinculin binding proteins in muscle and nonmuscle tissues with an electroblot-overlay technique.
    Biochem Biophys Res Commun. 1983 Nov 15;116(3):1026-32 PMID: 6418169
  10. An interaction between vinculin and talin.
    Nature. 1984 Apr 19-25;308(5961):744-6 PMID: 6425696
  11. Reconstitution of the purified platelet fibrinogen receptor. Fibrinogen binding properties of the glycoprotein IIb-IIIa complex.
    J Biol Chem. 1985 Sep 5;260(19):10698-707 PMID: 2993285
  12. Interaction of plasma membrane fibronectin receptor with talin--a transmembrane linkage.
    Nature. 1986 Apr 10-16;320(6062):531-3 PMID: 2938015
  13. Structure of integrin, a glycoprotein involved in the transmembrane linkage between fibronectin and actin.
    Cell. 1986 Jul 18;46(2):271-82 PMID: 3487386
  14. Fibronectin-binding properties of the purified platelet glycoprotein IIb-IIIa complex.
    J Biol Chem. 1986 Oct 25;261(30):14011-7 PMID: 3771519
  15. Studies on proteins that co-purify with smooth muscle vinculin: identification of immunologically related species in focal adhesions of nonmuscle and Z-lines of muscle cells.
    J Cell Biol. 1986 Oct;103(4):1483-94 PMID: 3095336
  16. Identification of a new protein localized at sites of cell-substrate adhesion.
    J Cell Biol. 1986 Nov;103(5):1679-87 PMID: 3536951
  17. Integrins: a family of cell surface receptors.
    Cell. 1987 Feb 27;48(4):549-54 PMID: 3028640
  18. Further characterization of a conserved actin-binding 27-kDa fragment of actinogelin and alpha-actinins and mapping of their binding sites on the actin molecule by chemical cross-linking.
    J Biol Chem. 1987 Apr 5;262(10):4717-23 PMID: 3558365
  19. Alpha-actinin is absent from the terminal segments of myofibrils and from subsarcolemmal densities in frog skeletal muscle.
    Exp Cell Res. 1987 Jun;170(2):469-82 PMID: 3496231
  20. The human fibroblast class II extracellular matrix receptor mediates platelet adhesion to collagen and is identical to the platelet glycoprotein Ia-IIa complex.
    J Biol Chem. 1988 Apr 5;263(10):4516-9 PMID: 2832397
  21. The complete sequence of dystrophin predicts a rod-shaped cytoskeletal protein.
    Cell. 1988 Apr 22;53(2):219-28 PMID: 3282674
  22. Antibodies to the conserved cytoplasmic domain of the integrin beta 1 subunit react with proteins in vertebrates, invertebrates, and fungi.
    J Cell Biol. 1988 May;106(5):1765-72 PMID: 3131349
  23. Novel tyrosine kinase substrates from Rous sarcoma virus-transformed cells are present in the membrane skeleton.
    J Cell Biol. 1989 Jun;108(6):2401-8 PMID: 2472406
  24. Fibulin, a novel protein that interacts with the fibronectin receptor beta subunit cytoplasmic domain.
    Cell. 1989 Aug 25;58(4):623-9 PMID: 2527614
  25. Identification of a neuronal laminin receptor: an Mr 200K/120K integrin heterodimer that binds laminin in a divalent cation-dependent manner.
    Neuron. 1988 Oct;1(8):713-25 PMID: 2978786
  26. Molecular genetics of Drosophila alpha-actinin: mutant alleles disrupt Z disc integrity and muscle insertions.
    J Cell Biol. 1990 Jun;110(6):1999-2011 PMID: 2112549
  27. Structural analysis of homologous repeated domains in alpha-actinin and spectrin.
    Int J Biol Macromol. 1989 Apr;11(2):81-90 PMID: 2489070
  28. Studies on the structure of myosin.
    J Mol Biol. 1962 Apr;4:293-308 PMID: 14466961
  29. X-ray diffraction evidence for alpha-helical coiled-coils in native muscle.
    J Mol Biol. 1963 May;6:423-32 PMID: 14022021
  30. Specific interaction of vinculin with alpha-actinin.
    Biochem Biophys Res Commun. 1987 Jul 31;146(2):554-60 PMID: 3113423
  31. Protein sequence of DMD gene is related to actin-binding domain of alpha-actinin.
    Cell. 1987 Oct 9;51(1):1 PMID: 3652206
  32. Amino acid sequence of the human fibronectin receptor.
    J Cell Biol. 1987 Sep;105(3):1183-90 PMID: 2958481
  33. New perspectives in cell adhesion: RGD and integrins.
    Science. 1987 Oct 23;238(4826):491-7 PMID: 2821619
  34. Purification of two smooth muscle glycoproteins related to integrin. Distribution in cultured chicken embryo fibroblasts.
    J Biol Chem. 1987 Dec 15;262(35):17189-99 PMID: 3500174
  35. Cell surface receptors for extracellular matrix molecules.
    Annu Rev Cell Biol. 1987;3:179-205 PMID: 2825736
  36. The Duchenne muscular dystrophy gene product is localized in sarcolemma of human skeletal muscle.
    Nature. 1988 Jun 2;333(6172):466-9 PMID: 3287171
  37. Immunostaining of skeletal and cardiac muscle surface membrane with antibody against Duchenne muscular dystrophy peptide.
    Nature. 1988 Jun 30;333(6176):861-3 PMID: 3290683
  38. Immunoelectron microscopic localization of dystrophin in myofibres.
    Nature. 1988 Jun 30;333(6176):863-6 PMID: 3290684
  39. The human laminin receptor is a member of the integrin family of cell adhesion receptors.
    Science. 1988 Sep 2;241(4870):1228-9 PMID: 2970671
  40. The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa.
    J Cell Biol. 1988 Nov;107(5):1881-91 PMID: 2846588
  41. Laminin receptor on platelets is the integrin VLA-6.
    Nature. 1988 Dec 1;336(6198):487-9 PMID: 2973567
  42. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton.
    Annu Rev Cell Biol. 1988;4:487-525 PMID: 3058164
  43. Some properties of purified skeletal muscle alpha-actinin.
    J Biol Chem. 1976 Nov 10;251(21):6860-70 PMID: 977599
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.2.721
Published
1990-08-00
Pages
721-9
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116186
Subset
IM
Grants
NCI NIH HHS · CA-08493 · United States
NIGMS NIH HHS · GM-29860 · United States
External Links
PubMed source DOI: 10.1083/jcb.111.2.721 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2