Home LiteratureArticle Details
PMID: 2110361 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 9 ·1990-05-00 ·Pages 3328-32

Kanner SB, Reynolds AB, Vines RR, Parsons JT

Abstract

Cellular transformation by oncogenic retroviruses encoding protein tyrosine kinases coincides with the tyrosine-specific phosphorylation of multiple protein substrates. Previous studies have shown that tyrosine phosphorylation of a protein of 120 kDa, p120, correlated with src transformation in chicken embryo fibroblasts. Additionally, we previously identified two phosphotyrosine-containing cellular proteins, p130 and p110, that formed stable complexes with activated variants of pp60src, the src-encoded tyrosine kinase. To study transformation-relevant tyrosine kinase substrates, we have generated monoclonal antibodies to individual tyrosine phosphoproteins, including p130, p120, p110, and five additional phosphoproteins (p210, p125, p118, p85, and p185/p64). These antibodies detected several of the same tyrosine phosphoproteins in chicken embryo fibroblasts transformed by avian retroviruses Y73 and CT10, encoding the yes and crk oncogenes, respectively. Protein substrates in mouse, rat, hamster, and human cells overexpressing activated variants of chicken pp60src were also detected by several of the monoclonal antibodies.

MeSH Terms
Animals Antibodies, Monoclonal Antigen-Antibody Complex Cell Line Cell Transformation, Neoplastic Cells, Cultured Chick Embryo Enzyme-Linked Immunosorbent Assay Humans Immunoblotting Immunoglobulin G Immunoglobulin M Oncogene Protein pp60(v-src)/genetics,metabolism Oncogenes Phosphoproteins/analysis Protein-Tyrosine Kinases/genetics,metabolism Tyrosine
Chemicals
Antibodies, Monoclonal Antigen-Antibody Complex Immunoglobulin G Immunoglobulin M Phosphoproteins Tyrosine Protein-Tyrosine Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kanner S B
Department of Microbiology and Cancer Center, University of Virginia Health Sciences Center, Charlottesville 22908.
Reynolds A B
Vines R R
Parsons J T
References (39)
39 references, click to expand
  1. The viral tyrosine protein kinases.
    Curr Top Microbiol Immunol. 1986;123:39-72 PMID: 2419041
  2. Non-catalytic domains of cytoplasmic protein-tyrosine kinases: regulatory elements in signal transduction.
    Oncogene. 1988 Nov;3(5):491-5 PMID: 3078956
  3. Protein tyrosine phosphorylation in the cell cycle of BALB/c 3T3 fibroblasts.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8191-5 PMID: 3464948
  4. Activation and suppression of pp60c-src transforming ability by mutation of its primary sites of tyrosine phosphorylation.
    Cell. 1987 Apr 10;49(1):65-73 PMID: 3103925
  5. Tyrosine phosphorylation regulates the biochemical and biological properties of pp60c-src.
    Cell. 1987 Apr 10;49(1):75-82 PMID: 3103926
  6. Cell transformation by pp60c-src mutated in the carboxy-terminal regulatory domain.
    Cell. 1987 Apr 10;49(1):83-91 PMID: 3103927
  7. Activation of the oncogenic potential of the avian cellular src protein by specific structural alteration of the carboxy terminus.
    EMBO J. 1987 Aug;6(8):2359-64 PMID: 2822389
  8. Tyrosine phosphorylations in vivo associated with v-fms transformation.
    Mol Cell Biol. 1988 Jan;8(1):176-85 PMID: 2447483
  9. Structural features of the carboxy terminus of p60c-src that are required for the regulation of its intrinsic kinase activity.
    Jpn J Cancer Res. 1987 Dec;78(12):1354-62 PMID: 3123438
  10. Sequence similarity of phospholipase C with the non-catalytic region of src.
    Nature. 1988 Mar 17;332(6161):269-72 PMID: 2831461
  11. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  12. Continuous cultures of fused cells secreting antibody of predefined specificity.
    Nature. 1975 Aug 7;256(5517):495-7 PMID: 1172191
  13. Characterization and use of monoclonal antibodies for isolation of phosphotyrosyl proteins from retrovirus-transformed cells and growth factor-stimulated cells.
    Mol Cell Biol. 1983 Aug;3(8):1343-52 PMID: 6194425
  14. Monoclonal antibodies to Rous sarcoma virus pp60src react with enzymatically active cellular pp60src of avian and mammalian origin.
    J Virol. 1984 Aug;51(2):272-82 PMID: 6205164
  15. Recognition of two Dermatophagoides pteronyssinus-specific epitopes on antigen P1 by using monoclonal antibodies: binding to each epitope can be inhibited by serum from dust mite-allergic patients.
    J Immunol. 1984 Nov;133(5):2488-95 PMID: 6207232
  16. A novel viral oncogene with structural similarity to phospholipase C.
    Nature. 1988 Mar 17;332(6161):272-5 PMID: 2450282
  17. Nonmyristoylated p60v-src fails to phosphorylate proteins of 115-120 kDa in chicken embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2608-12 PMID: 2451829
  18. Monoclonal antibodies to phosphotyrosine.
    J Immunol Methods. 1988 May 9;109(2):277-85 PMID: 2452204
  19. Identification of multiple novel polypeptide substrates of the v-src, v-yes, v-fps, v-ros, and v-erb-B oncogenic tyrosine protein kinases utilizing antisera against phosphotyrosine.
    Oncogene. 1988 Apr;2(4):305-15 PMID: 2452398
  20. Activation of pp60c-src tyrosine kinase specific activity in tumor-derived Syrian hamster embryo cells.
    Oncogene. 1988 Apr;2(4):327-35 PMID: 2452399
  21. Nerve growth factor induces protein-tyrosine phosphorylation.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6788-91 PMID: 2457918
  22. Identification and subcellular localization of proteins that are rapidly phosphorylated in tyrosine in response to colony-stimulating factor 1.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):8062-6 PMID: 2460861
  23. Human cdc2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate.
    Nature. 1988 Dec 22-29;336(6201):738-44 PMID: 2462672
  24. Phosphorylation of cellular proteins in Rous sarcoma virus-infected cells: analysis by use of anti-phosphotyrosine antibodies.
    Mol Cell Biol. 1988 Aug;8(8):3035-42 PMID: 2463469
  25. Platelet tyrosine-specific protein phosphorylation is regulated by thrombin.
    Mol Cell Biol. 1988 Sep;8(9):3603-10 PMID: 2464741
  26. Thrombin treatment induces rapid changes in tyrosine phosphorylation in platelets.
    Proc Natl Acad Sci U S A. 1989 Feb;86(3):901-5 PMID: 2464830
  27. Tyrosine-specific protein phosphorylation is regulated by glycoprotein IIb-IIIa in platelets.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2234-8 PMID: 2928328
  28. Novel tyrosine phosphorylations accompany the activation of pp60c-src during chemical carcinogenesis.
    Oncogene. 1989 Mar;4(3):295-300 PMID: 2468123
  29. Transformation-specific tyrosine phosphorylation of a novel cellular protein in chicken cells expressing oncogenic variants of the avian cellular src gene.
    Mol Cell Biol. 1989 Feb;9(2):629-38 PMID: 2469003
  30. Immunoaffinity purification of tyrosine-phosphorylated cellular proteins.
    J Immunol Methods. 1989 Jun 2;120(1):115-24 PMID: 2471744
  31. Phospholipase C-gamma is a substrate for the PDGF and EGF receptor protein-tyrosine kinases in vivo and in vitro.
    Cell. 1989 Jun 30;57(7):1109-22 PMID: 2472219
  32. Novel tyrosine kinase substrates from Rous sarcoma virus-transformed cells are present in the membrane skeleton.
    J Cell Biol. 1989 Jun;108(6):2401-8 PMID: 2472406
  33. Direct activation of the serine/threonine kinase activity of Raf-1 through tyrosine phosphorylation by the PDGF beta-receptor.
    Cell. 1989 Aug 25;58(4):649-57 PMID: 2475255
  34. Platelet-derived growth factor induces rapid and sustained tyrosine phosphorylation of phospholipase C-gamma in quiescent BALB/c 3T3 cells.
    Mol Cell Biol. 1989 Jul;9(7):2934-43 PMID: 2550789
  35. Stable association of activated pp60src with two tyrosine-phosphorylated cellular proteins.
    Mol Cell Biol. 1989 Sep;9(9):3951-8 PMID: 2476666
  36. PDGF induction of tyrosine phosphorylation of GTPase activating protein.
    Nature. 1989 Dec 7;342(6250):711-4 PMID: 2480526
  37. Phosphorylation of GAP and GAP-associated proteins by transforming and mitogenic tyrosine kinases.
    Nature. 1990 Jan 25;343(6256):377-81 PMID: 1689011
  38. A 42-kD tyrosine kinase substrate linked to chromaffin cell secretion exhibits an associated MAP kinase activity and is highly related to a 42-kD mitogen-stimulated protein in fibroblasts.
    J Cell Biol. 1990 Mar;110(3):731-42 PMID: 1689732
  39. Phosphatidylinositol metabolism and polyoma-mediated transformation.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3624-8 PMID: 2424008
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.9.3328
Published
1990-05-00
Pages
3328-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53893
Subset
IM
Grants
NCI NIH HHS · CA08316 · United States
NCI NIH HHS · CA29243 · United States
NCI NIH HHS · CA40042 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.9.3328 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2