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PMID: 2457918 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nerve growth factor induces protein-tyrosine phosphorylation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 18 ·1988-09-00 ·Pages 6788-91

Maher PA

Abstract

When the sympathetic nerve-like cell line PC12 is exposed to nerve growth factor (NGF), there is a rapid and transient phosphorylation of tyrosine residues in cellular proteins, as demonstrated by immunoblotting of cell extracts with high-affinity polyclonal antibodies specific for phosphotyrosine residues. Epidermal growth factor (EGF), which does not cause the morphological differentiation of PC12 cells that is produced by NGF, also induces protein-tyrosine phosphorylation. The methyltransferase inhibitor, 5'-methylthioadenosine, which is known to block the NGF-mediated morphological differentiation of PC12 cells, also inhibits the induction of protein-tyrosine phosphorylation by NGF. 5'-Methylthioadenosine has no effect, however, on EGF-stimulated phosphorylation of tyrosine residues in cellular proteins. In addition, low temperature markedly slows the rate of protein-tyrosine phosphorylation stimulated by NGF, but it has no effect on the time course of protein-tyrosine phosphorylation induced by EGF. These data suggest that NGF and EGF induce protein-tyrosine phosphorylation in PC12 cells by different mechanisms.

MeSH Terms
Adrenal Gland Neoplasms/enzymology Animals Bucladesine/pharmacology Cell Line Epidermal Growth Factor/pharmacology Immunosorbent Techniques Nerve Growth Factors/metabolism Pheochromocytoma/enzymology Phosphorylation Phosphotyrosine Protein-Tyrosine Kinases/metabolism Rats Tyrosine/analogs & derivatives,metabolism
Chemicals
Nerve Growth Factors Phosphotyrosine Tyrosine Epidermal Growth Factor Bucladesine Protein-Tyrosine Kinases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Maher P A
Department of Biology, University of California, San Diego, La Jolla 92093.
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38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.18.6788
Published
1988-09-00
Pages
6788-91
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282063
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.18.6788 PMC Full Text View journal details
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