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PMID: 2118142 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Paxillin: a new vinculin-binding protein present in focal adhesions.

The Journal of cell biology ·Vol. 111 ·No. 3 ·1990-09-00 ·Pages 1059-68

Turner CE, Glenney JR, Burridge K

Abstract

The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells. Paxillin purified from chicken gizzard smooth muscle migrates as a diffuse band on SDS-PAGE gels with a molecular mass of 65-70 kD. It is a protein of multiple isoforms with pIs ranging from 6.31 to 6.85. Using purified paxillin, we have demonstrated a specific interaction in vitro with another focal adhesion protein, vinculin. Cleavage of vinculin with Staphylococcus aureus V8 protease results in the generation of two fragments of approximately 85 and 27 kD. Unlike talin, which binds to the large vinculin fragment, paxillin was found to bind to the small vinculin fragment, which represents the rod domain of the molecule. Together with the previous observation that paxillin is a major substrate of pp60src in Rous sarcoma virus-transformed cells (Glenney, J. R., and L. Zokas. 1989. J. Cell Biol. 108:2401-2408), this interaction with vinculin suggests paxillin may be a key component in the control of focal adhesion organization.

MeSH Terms
Animals Carrier Proteins/isolation & purification,physiology Cell Adhesion/physiology Cell Adhesion Molecules/isolation & purification,physiology Cells, Cultured Chickens Cytoskeletal Proteins/metabolism Fluorescent Antibody Technique Gizzard, Avian Muscle Proteins/isolation & purification,physiology Muscle, Smooth/physiology Vinculin
Chemicals
Carrier Proteins Cell Adhesion Molecules Cytoskeletal Proteins Muscle Proteins Vinculin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Turner C E
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599-7090.
Glenney J R
Burridge K
References (54)
54 references, click to expand
  1. Reorganization of alpha-actinin and vinculin induced by a phorbol ester in living cells.
    J Cell Biol. 1986 Apr;102(4):1430-8 PMID: 3082892
  2. Rous sarcoma virus transforming protein lacking myristic acid phosphorylates known polypeptide substrates without inducing transformation.
    Cell. 1986 Apr 11;45(1):105-12 PMID: 3006923
  3. Phosphorylation of the fibronectin receptor complex in cells transformed by oncogenes that encode tyrosine kinases.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6470-4 PMID: 3018734
  4. Studies on proteins that co-purify with smooth muscle vinculin: identification of immunologically related species in focal adhesions of nonmuscle and Z-lines of muscle cells.
    J Cell Biol. 1986 Oct;103(4):1483-94 PMID: 3095336
  5. Identification of a new protein localized at sites of cell-substrate adhesion.
    J Cell Biol. 1986 Nov;103(5):1679-87 PMID: 3536951
  6. Integrins: a family of cell surface receptors.
    Cell. 1987 Feb 27;48(4):549-54 PMID: 3028640
  7. Purification and assay of vinculin, metavinculin, and talin.
    Methods Enzymol. 1986;134:69-77 PMID: 3102903
  8. Phosphorylation of talin at tyrosine in Rous sarcoma virus-transformed cells.
    Mol Cell Biol. 1987 Jan;7(1):371-8 PMID: 3031468
  9. Properties of talin from chicken gizzard smooth muscle.
    J Biol Chem. 1987 Jun 5;262(16):7790-5 PMID: 3108258
  10. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  11. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  12. Alpha-actinin: immunofluorescent localization of a muscle structural protein in nonmuscle cells.
    Cell. 1975 Nov;6(3):289-98 PMID: 802682
  13. A 130K protein from chicken gizzard: its localization at the termini of microfilament bundles in cultured chicken cells.
    Cell. 1979 Sep;18(1):193-205 PMID: 574428
  14. A rapid purification of alpha-actinin, filamin, and a 130,000-dalton protein from smooth muscle.
    J Biol Chem. 1980 Feb 10;255(3):1194-9 PMID: 7356657
  15. Microinjection and localization of a 130K protein in living fibroblasts: a relationship to actin and fibronectin.
    Cell. 1980 Mar;19(3):587-95 PMID: 6988083
  16. Fimbrin, a new microfilament-associated protein present in microvilli and other cell surface structures.
    J Cell Biol. 1980 Jul;86(1):335-40 PMID: 6998986
  17. Vinculin: a cytoskeletal target of the transforming protein of Rous sarcoma virus.
    Cell. 1981 Apr;24(1):165-74 PMID: 6263485
  18. F-actin binding and bundling properties of fimbrin, a major cytoskeletal protein of microvillus core filaments.
    J Biol Chem. 1981 Sep 10;256(17):9283-8 PMID: 6894925
  19. Fimbrin is a cytoskeletal protein that crosslinks F-actin in vitro.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6849-53 PMID: 6947259
  20. Immunoelectron microscope studies of membrane-microfilament interactions: distributions of alpha-actinin, tropomyosin, and vinculin in intestinal epithelial brush border and chicken gizzard smooth muscle cells.
    J Cell Biol. 1981 Dec;91(3 Pt 1):614-28 PMID: 6799520
  21. Brain spectrin, a membrane-associated protein related in structure and function to erythrocyte spectrin.
    Nature. 1982 Sep 9;299(5879):126-31 PMID: 7110333
  22. A new protein of adhesion plaques and ruffling membranes.
    J Cell Biol. 1983 Aug;97(2):359-67 PMID: 6684120
  23. Vinculin, a cytoskeletal substrate of protein kinase C.
    J Biol Chem. 1983 Oct 10;258(19):11423-6 PMID: 6225775
  24. Detection of vinculin-binding proteins with an 125I-vinculin gel overlay technique.
    J Cell Biol. 1983 Oct;97(4):1283-7 PMID: 6413514
  25. Detection of high molecular weight vinculin binding proteins in muscle and nonmuscle tissues with an electroblot-overlay technique.
    Biochem Biophys Res Commun. 1983 Nov 15;116(3):1026-32 PMID: 6418169
  26. Vinculin phosphorylation in response to calcium and phorbol esters in intact cells.
    J Biol Chem. 1984 Apr 25;259(8):5264-70 PMID: 6325428
  27. An interaction between vinculin and talin.
    Nature. 1984 Apr 19-25;308(5961):744-6 PMID: 6425696
  28. A tumor promoter induces rapid and coordinated reorganization of actin and vinculin in cultured cells.
    J Cell Biol. 1984 Sep;99(3):1045-59 PMID: 6206076
  29. Interaction of tumour promoters with epithelial cells in culture. An immunofluorescence study.
    Exp Cell Res. 1985 Oct;160(2):259-74 PMID: 3930274
  30. Interaction of iodinated vinculin, metavinculin and alpha-actinin with cytoskeletal proteins.
    FEBS Lett. 1987 Aug 17;220(2):291-4 PMID: 3111888
  31. Specific interaction of vinculin with alpha-actinin.
    Biochem Biophys Res Commun. 1987 Jul 31;146(2):554-60 PMID: 3113423
  32. New perspectives in cell adhesion: RGD and integrins.
    Science. 1987 Oct 23;238(4826):491-7 PMID: 2821619
  33. Colocalization of calcium-dependent protease II and one of its substrates at sites of cell adhesion.
    Cell. 1987 Nov 20;51(4):569-77 PMID: 2824061
  34. Cell surface receptors for extracellular matrix molecules.
    Annu Rev Cell Biol. 1987;3:179-205 PMID: 2825736
  35. Tyrosine phosphorylated proteins in different tissues during chick embryo development.
    J Cell Biol. 1988 May;106(5):1747-55 PMID: 2453521
  36. cDNA-derived sequence of chicken embryo vinculin.
    Proc Natl Acad Sci U S A. 1988 Nov;85(22):8535-9 PMID: 3141928
  37. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton.
    Annu Rev Cell Biol. 1988;4:487-525 PMID: 3058164
  38. Primary sequence and domain structure of chicken vinculin.
    Biochem J. 1989 Apr 15;259(2):453-61 PMID: 2497736
  39. Identification of two distinct functional domains on vinculin involved in its association with focal contacts.
    J Cell Biol. 1989 Jun;108(6):2383-93 PMID: 2500446
  40. Novel tyrosine kinase substrates from Rous sarcoma virus-transformed cells are present in the membrane skeleton.
    J Cell Biol. 1989 Jun;108(6):2401-8 PMID: 2472406
  41. The role of phosphorylation and limited proteolytic cleavage of talin and vinculin in the disruption of focal adhesion integrity.
    J Biol Chem. 1989 Jul 15;264(20):11938-44 PMID: 2545690
  42. Detection of metavinculin in human platelets using a modified talin overlay assay.
    Eur J Cell Biol. 1989 Jun;49(1):202-6 PMID: 2503380
  43. Association of type 3 protein kinase C with focal contacts in rat embryo fibroblasts.
    J Cell Biol. 1989 Aug;109(2):697-704 PMID: 2668303
  44. Identification of a talin binding site in the cytoskeletal protein vinculin.
    J Cell Biol. 1989 Dec;109(6 Pt 1):2917-27 PMID: 2512301
  45. Purification and characterization of an 85 kDa talin-binding fragment of vinculin.
    Cell Motil Cytoskeleton. 1990;15(1):41-50 PMID: 2104778
  46. Functional studies of the domains of talin.
    J Cell Biol. 1990 May;110(5):1635-44 PMID: 2110569
  47. The adhesion plaque protein, talin, is phosphorylated in vivo in chicken embryo fibroblasts exposed to a tumor-promoting phorbol ester.
    Cell Regul. 1990 Jan;1(2):227-36 PMID: 2129221
  48. Junctional complexes in various epithelia.
    J Cell Biol. 1963 May;17:375-412 PMID: 13944428
  49. Molecular heterogeneity of adherens junctions.
    J Cell Biol. 1985 Oct;101(4):1523-31 PMID: 3930512
  50. Electron microscopy of rotary shadowed vinculin and vinculin complexes.
    J Mol Biol. 1985 Aug 5;184(3):543-5 PMID: 3930754
  51. Molecular shape and self-association of vinculin and metavinculin.
    J Cell Biochem. 1985;29(1):31-6 PMID: 3932372
  52. Phosphorylation of the cytoskeletal protein talin by protein kinase C.
    Biochem Biophys Res Commun. 1986 Feb 13;134(3):1276-83 PMID: 3080999
  53. A re-examination of the interaction of vinculin with actin.
    J Cell Biol. 1986 Mar;102(3):1085-92 PMID: 3005334
  54. Talin is phosphorylated on tyrosine in chicken embryo fibroblasts transformed by Rous sarcoma virus.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5507-11 PMID: 2426700
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.3.1059
Published
1990-09-00
Pages
1059-68
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116264
Subset
IM
Grants
NIGMS NIH HHS · GM-29860 · United States
NIGMS NIH HHS · GM-32866 · United States
External Links
PubMed source DOI: 10.1083/jcb.111.3.1059 PMC Full Text View journal details
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