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PMID: 7799929 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two domains of p53 interact with the TATA-binding protein, and the adenovirus 13S E1A protein disrupts the association, relieving p53-mediated transcriptional repression.

Molecular and cellular biology ·Vol. 15 ·No. 1 ·1995-01-00 ·Pages 227-34

Horikoshi N, Usheva A, Chen J, Levine AJ, Weinmann R, Shenk T

Abstract

The tumor suppressor gene product p53 can activate and repress transcription. Both transcriptional activation and repression are thought to involve the direct interaction of p53 with the basal transcriptional machinery. Previous work has demonstrated an in vitro interaction between p53 and the TATA-binding protein that requires amino acids 20 to 57 of p53 and amino acids 220 to 271 of the TATA-binding protein. The present results show that a 75-amino-acid segment from the carboxy terminus of p53 also can bind to the TATA-binding protein in vitro, and this interaction requires amino acids 217 to 268 of the TATA-binding protein, essentially the same domain that is required for interaction with the amino-terminal domain of p53. A carboxy-terminal segment of p53 can mediate repression when bound to DNA as a GAL4-p53 fusion protein. The amino- and carboxy-terminal p53 interactions occur within the domain on the TATA-binding protein to which the adenovirus 13S E1A oncoprotein has previously been shown to bind. The 13S E1A oncoprotein can dissociate the complex formed between the carboxy-terminal domain of p53 and the TATA-binding protein and relieve p53-mediated transcriptional repression. These results demonstrate that two independent domains of p53 can potentially interact with the TATA-binding protein, and they define a mechanism--relief of repression--by which the 13S E1A oncoprotein can activate transcription through the TATA motif.

MeSH Terms
Adenovirus E1A Proteins/metabolism Adenoviruses, Human/genetics Base Sequence DNA Primers/chemistry DNA-Binding Proteins/chemistry,metabolism Gene Expression Regulation, Viral Humans In Vitro Techniques Molecular Sequence Data Mutagenesis, Site-Directed Repressor Proteins/metabolism Structure-Activity Relationship TATA Box TATA-Box Binding Protein Transcription Factors/chemistry,metabolism Transcription, Genetic Tumor Suppressor Protein p53/chemistry,metabolism
Chemicals
Adenovirus E1A Proteins DNA Primers DNA-Binding Proteins Repressor Proteins TATA-Box Binding Protein Transcription Factors Tumor Suppressor Protein p53
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Horikoshi N
Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, New Jersey 08544-1014.
Usheva A
Chen J
Levine A J
Weinmann R
Shenk T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.1.227
Published
1995-01-00
Pages
227-34
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231940
Subset
IM
Grants
NCI NIH HHS · CA41086 · United States
NCI NIH HHS · CA44466 · United States
External Links
PubMed source DOI: 10.1128/MCB.15.1.227 PMC Full Text View journal details
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