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PMID: 1705714 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Growth suppression induced by wild-type p53 protein is accompanied by selective down-regulation of proliferating-cell nuclear antigen expression.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 5 ·1991-03-01 ·Pages 1958-62

Mercer WE, Shields MT, Lin D, Appella E, Ullrich SJ

Abstract

The p53 gene is a frequent target of mutation in a wide variety of human cancers. Previously, it was reported that conditional expression of wild-type p53 protein in a cell line (GM47.23) derived from a human glioblastoma multiform tumor had a negative effect on cell proliferation. We have now investigated the effect that induction of wild-type p53 protein in this cell line has on the expression of the proliferating-cell nuclear antigen gene. The proliferating-cell nuclear antigen gene encodes a nuclear protein that is an auxiliary factor of DNA polymerase delta and part of the DNA replication machinery of the cell. We show that inhibition of cell cycle progression into S-phase after induction of wild-type p53 protein is accompanied by selective down-regulation of proliferating-cell nuclear antigen mRNA and protein expression.

MeSH Terms
Antigens, Neoplasm/genetics Blotting, Northern Cell Line Fluorescent Antibody Technique Humans Nuclear Proteins/analysis,genetics Plasmids Proliferating Cell Nuclear Antigen RNA/genetics,isolation & purification RNA, Messenger/genetics Restriction Mapping Transfection Tumor Suppressor Protein p53/analysis,genetics,metabolism
Chemicals
Antigens, Neoplasm Nuclear Proteins Proliferating Cell Nuclear Antigen RNA, Messenger Tumor Suppressor Protein p53 RNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mercer W E
Department of Pathology, Temple University School of Medicine, Philadelphia, PA 19140.
Shields M T
Lin D
Appella E
Ullrich S J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.5.1958
Published
1991-03-01
Pages
1958-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51145
Subset
IM
Grants
NCI NIH HHS · CA 09644 · United States
NCI NIH HHS · CA42866 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.5.1958 PMC Full Text View journal details
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