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PMID: 2144364 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcriptional activation by wild-type but not transforming mutants of the p53 anti-oncogene.

Science (New York, N.Y.) ·Vol. 249 ·No. 4972 ·1990-08-31 ·Pages 1049-51

Raycroft L, Wu HY, Lozano G

Abstract

The protein encoded by the wild-type p53 proto-oncogene has been shown to suppress transformation, whereas certain mutations that alter p53 become transformation competent. Fusion proteins between p53 and the GAL4 DNA binding domain were made to anchor p53 to a DNA target sequence and to allow measurement of transcriptional activation of a reporter plasmid. The wild-type p53 stimulated transcription in this assay, but two transforming mutations in p53 were unable to act as transcriptional activators. Therefore, p53 can activate transcription, and transformation-activating mutations result in a loss of function of the p53 protein. The inability of the p53 mutant proteins to activate transcription may enable them to be transformation competent.

MeSH Terms
Base Sequence Cell Transformation, Neoplastic Gene Expression Regulation HeLa Cells/metabolism Humans Molecular Sequence Data Mutation Nuclear Proteins/genetics Oligonucleotide Probes Oncogene Proteins/genetics Phosphoproteins/genetics Proto-Oncogene Mas Proto-Oncogenes RNA, Messenger/genetics Suppression, Genetic Transcription Factors/genetics Transcription, Genetic Tumor Suppressor Protein p53
Chemicals
MAS1 protein, human Nuclear Proteins Oligonucleotide Probes Oncogene Proteins Phosphoproteins Proto-Oncogene Mas RNA, Messenger Transcription Factors Tumor Suppressor Protein p53
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Raycroft L
University of Texas, M. D. Anderson Cancer Center, Department of Molecular Genetics, Houston 77030.
Wu H Y
Lozano G
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33 references, click to expand
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.2144364
Published
1990-08-31
Pages
1049-51
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC2935288
Subset
IM
Grants
NCI NIH HHS · R01 CA047296 · United States
NCI NIH HHS · R01 CA047296-12 · United States
NCI NIH HHS · CA47296 · United States
NCI NIH HHS · CA16672 · United States
External Links
PubMed source DOI: 10.1126/science.2144364 PMC Full Text View journal details
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