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PMID: 2233717 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

p53 functions as a cell cycle control protein in osteosarcomas.

Molecular and cellular biology ·Vol. 10 ·No. 11 ·1990-11-00 ·Pages 5772-81

Diller L, Kassel J, Nelson CE, Gryka MA, Litwak G, Gebhardt M, Bressac B, Ozturk M, Baker SJ, Vogelstein B

Abstract

Mutations in the p53 gene have been associated with a wide range of human tumors, including osteosarcomas. Although it has been shown that wild-type p53 can block the ability of E1a and ras to cotransform primary rodent cells, it is poorly understood why inactivation of the p53 gene is important for tumor formation. We show that overexpression of the gene encoding wild-type p53 blocks the growth of osteosarcoma cells. The growth arrest was determined to be due to an inability of the transfected cells to progress into S phase. This suggests that the role of the p53 gene as an antioncogene may be in controlling the cell cycle in a fashion analogous to the check-point control genes in Saccharomyces cerevisiae.

MeSH Terms
Amino Acid Sequence Cell Cycle Cell Line Flow Cytometry Fluorescent Antibody Technique Humans Molecular Sequence Data Mutagenesis, Site-Directed Osteosarcoma/pathology Polymerase Chain Reaction Restriction Mapping Transfection Tumor Suppressor Protein p53/genetics,physiology
Chemicals
Tumor Suppressor Protein p53
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Diller L
Massachusetts General Hospital Cancer Center, Charlestown 02129.
Kassel J
Nelson C E
Gryka M A
Litwak G
Gebhardt M
Bressac B
Ozturk M
Baker S J
Vogelstein B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.11.5772-5781.1990
Published
1990-11-00
Pages
5772-81
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361354
Subset
IM
Grants
NCI NIH HHS · 5T32CA09172-16 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.11.5772-5781.1990 PMC Full Text View journal details
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