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PMID: 8090749 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Relief of p53-mediated transcriptional repression by the adenovirus E1B 19-kDa protein or the cellular Bcl-2 protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 19 ·1994-09-13 ·Pages 8940-4

Shen Y, Shenk T

Abstract

The p53 tumor suppressor gene product is a transcriptional regulatory protein. It activates transcription from promoters that contain a p53 DNA binding site but represses many promoters that lack its binding site. High-level expression of wild-type p53 can induce apoptosis in certain cell types, and this activity can be blocked by the adenovirus E1B 19-kDa oncoprotein or by the cellular Bcl-2 oncoprotein. Here we report that p53-mediated repression of promoters that lack a p53 binding site is abrogated by the E1B 19-kDa protein or Bcl-2 oncoprotein. In contrast, transcriptional activation by p53 still occurs in the presence of either protein. The fact that two oncoproteins capable of preventing p53-mediated apoptosis also block transcriptional repression by p53 raises the possibility that p53 might induce apoptosis, at least in part, by repressing transcription.

MeSH Terms
Adenovirus E1B Proteins/genetics Apoptosis Cell Nucleus/metabolism Gene Expression Regulation HeLa Cells Humans In Vitro Techniques Promoter Regions, Genetic Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-bcl-2 Repressor Proteins/genetics Transcription, Genetic Tumor Suppressor Protein p53/genetics
Chemicals
Adenovirus E1B Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Repressor Proteins Tumor Suppressor Protein p53
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shen Y
Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, NJ 08544-1014.
Shenk T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.19.8940
Published
1994-09-13
Pages
8940-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44722
Subset
IM
Grants
NCI NIH HHS · CA41086 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.19.8940 PMC Full Text View journal details
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