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PMID: 1409626 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Growth arrest induced by wild-type p53 protein blocks cells prior to or near the restriction point in late G1 phase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 19 ·1992-10-01 ·Pages 9210-4

Lin D, Shields MT, Ullrich SJ, Appella E, Mercer WE

Abstract

Conditional expression of wild-type (wt) p53 protein in a glioblastoma tumor cell line has been shown to be growth inhibitory. We have now more precisely localized the position in the cell cycle where growth arrest occurs. We show that growth arrest occurs prior to or near the restriction point in late G1 phase of the cell cycle. The effect of wt p53 protein on the expression of four immediate-early genes (c-FOS, c-JUN, JUN-B, and c-MYC), one delayed-early gene (ornithine decarboxylase), and two late-G1/S-phase genes (B-MYB and DNA polymerase alpha) was also examined. Of this subset of growth response genes, only the expression of B-MYB and DNA polymerase alpha was significantly repressed. The possibility that decreased expression of B-MYB may be an important component of growth arrest mediated by wt p53 protein is discussed.

Related Genes
JUN-B c-FOS c-JUN c-MYC
MeSH Terms
Blotting, Northern Cell Cycle/drug effects Cell Division/drug effects Cycloheximide/pharmacology DNA Polymerase II/genetics Dexamethasone/pharmacology G1 Phase/drug effects,physiology Genes, fos/drug effects Genes, jun/drug effects Genes, myc/drug effects Glioma Humans Ornithine Decarboxylase/genetics RNA, Messenger/genetics,metabolism RNA, Neoplasm/genetics,isolation & purification Tumor Cells, Cultured Tumor Suppressor Protein p53/genetics,physiology
Chemicals
RNA, Messenger RNA, Neoplasm Tumor Suppressor Protein p53 Dexamethasone Cycloheximide DNA Polymerase II Ornithine Decarboxylase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lin D
Department of Microbiology and Immunology, Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107.
Shields M T
Ullrich S J
Appella E
Mercer W E
References (38)
38 references, click to expand
  1. A c-myb antisense oligodeoxynucleotide inhibits normal human hematopoiesis in vitro.
    Science. 1988 Dec 2;242(4883):1303-6 PMID: 2461588
  2. Nucleotide sequence of cloned cDNA of human c-myc oncogene.
    Nature. 1983 Jun 23-29;303(5919):725-8 PMID: 6304538
  3. G1/S transition in normal human T-lymphocytes requires the nuclear protein encoded by c-myb.
    Science. 1989 Jul 14;245(4914):180-3 PMID: 2665077
  4. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.
    Science. 1987 Dec 4;238(4832):1386-92 PMID: 2825349
  5. Protooncogene expression during the cell cycle.
    Lab Invest. 1986 Apr;54(4):365-76 PMID: 3007866
  6. A gene activated by growth factors is related to the oncogene v-jun.
    Proc Natl Acad Sci U S A. 1988 Mar;85(5):1487-91 PMID: 3422745
  7. Molecules involved in proliferation of normal and cancer cells: presidential address.
    Cancer Res. 1987 Mar 15;47(6):1488-91 PMID: 3545446
  8. Expression of thymidine kinase and dihydrofolate reductase genes in mammalian ts mutants of the cell cycle.
    J Biol Chem. 1985 Mar 25;260(6):3269-74 PMID: 3838311
  9. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  10. Correlation between cell cycle duration and RNA content.
    J Cell Physiol. 1979 Sep;100(3):425-38 PMID: 489667
  11. Human wild-type p53 adopts a unique conformational and phosphorylation state in vivo during growth arrest of glioblastoma cells.
    Oncogene. 1992 Aug;7(8):1635-43 PMID: 1630823
  12. Inhibition of T-cell proliferation by a MYB antisense oligomer is accompanied by selective down-regulation of DNA polymerase alpha expression.
    Proc Natl Acad Sci U S A. 1990 Aug;87(15):5963-7 PMID: 1696013
  13. Effect of the myb gene product on expression of the PCNA gene in fibroblasts.
    Oncogene. 1991 Jun;6(6):887-94 PMID: 1712444
  14. Negative growth regulation in a glioblastoma tumor cell line that conditionally expresses human wild-type p53.
    Proc Natl Acad Sci U S A. 1990 Aug;87(16):6166-70 PMID: 2143581
  15. DNA binding activity and transcriptional activator function of the human B-myb protein compared with c-MYB.
    J Biol Chem. 1990 Jun 5;265(16):9280-4 PMID: 2160970
  16. p53 functions as a cell cycle control protein in osteosarcomas.
    Mol Cell Biol. 1990 Nov;10(11):5772-81 PMID: 2233717
  17. A comparison of cell cycle-related changes in postmitotic and quiescent AF8 cells as measured by cytofluorometry after acridine orange staining.
    Cancer Res. 1978 Aug;38(8):2514-8 PMID: 667846
  18. Temperature sensitive mutants of BHK cells affected in cell cycle progression.
    J Cell Physiol. 1977 Sep;92(3):425-36 PMID: 903382
  19. Repression of the interleukin 6 gene promoter by p53 and the retinoblastoma susceptibility gene product.
    Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7605-9 PMID: 1652755
  20. Tumor suppressor genes.
    Science. 1991 Nov 22;254(5035):1138-46 PMID: 1659741
  21. Growth suppression induced by wild-type p53 protein is accompanied by selective down-regulation of proliferating-cell nuclear antigen expression.
    Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1958-62 PMID: 1705714
  22. Wild-type p53 can down-modulate the activity of various promoters.
    Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):9979-83 PMID: 1946467
  23. Suppression of human colorectal carcinoma cell growth by wild-type p53.
    Science. 1990 Aug 24;249(4971):912-5 PMID: 2144057
  24. Binding of the c-myb proto-oncogene product to the simian virus 40 enhancer stimulates transcription.
    J Biol Chem. 1990 Feb 25;265(6):3479-83 PMID: 2154480
  25. Wild type human p53 is antiproliferative in SV40-transformed hamster cells.
    Oncogene. 1990 Jul;5(7):973-80 PMID: 2165234
  26. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  27. Cell cycle analysis of human peripheral blood T lymphocytes in long-term culture.
    Exp Cell Res. 1987 Nov;173(1):70-9 PMID: 2445595
  28. The p53 proto-oncogene can act as a suppressor of transformation.
    Cell. 1989 Jun 30;57(7):1083-93 PMID: 2525423
  29. An oligomer complementary to c-myb-encoded mRNA inhibits proliferation of human myeloid leukemia cell lines.
    Proc Natl Acad Sci U S A. 1989 May;86(9):3379-83 PMID: 2541445
  30. Transcriptional activation by the v-myb oncogene and its cellular progenitor, c-myb.
    Cell. 1989 Jul 14;58(1):85-93 PMID: 2665942
  31. G1 events and regulation of cell proliferation.
    Science. 1989 Nov 3;246(4930):603-8 PMID: 2683075
  32. Regulation of the proliferating cell nuclear antigen cyclin and thymidine kinase mRNA levels by growth factors.
    J Biol Chem. 1988 Jul 25;263(21):10175-9 PMID: 2899075
  33. Growth-dependent expression of human Mr 53,000 tumor antigen messenger RNA in normal and neoplastic cells.
    Cancer Res. 1986 Nov;46(11):5738-42 PMID: 3019534
  34. Isolation of human cDNA clones of myb-related genes, A-myb and B-myb.
    Nucleic Acids Res. 1988 Dec 9;16(23):11075-89 PMID: 3060855
  35. Gene expression during the mammalian cell cycle.
    Biochim Biophys Acta. 1986 Oct 28;865(2):83-125 PMID: 3533155
  36. TPA-induction of c-fos mRNA during the cell cycle of WI-38 human fibroblasts.
    Biochem Biophys Res Commun. 1987 Sep 15;147(2):716-23 PMID: 3632695
  37. Expression of the p53 protein during the cell cycle of human peripheral blood lymphocytes.
    Exp Cell Res. 1985 Sep;160(1):31-46 PMID: 3930272
  38. Wild-type p53 can inhibit oncogene-mediated focus formation.
    Proc Natl Acad Sci U S A. 1989 Nov;86(22):8763-7 PMID: 2530586
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.19.9210
Published
1992-10-01
Pages
9210-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50095
Subset
IM
Grants
NCI NIH HHS · CA 09644 · United States
NCI NIH HHS · CA 42866 · United States
NCRR NIH HHS · SO7 RR0 5417 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.19.9210 PMC Full Text View journal details
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