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PMID: 6761548 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Genetic studies on the beta subunit of Escherichia coli RNA polymerase. I. The effect of known, single amino acid substitutions in an essential protein.

Molecular & general genetics : MGG ·Vol. 188 ·No. 3 ·1982-00-00 ·Pages 399-404

Nene V, Glass RE

Abstract

The use of five different nonsense suppressors, in conjunction with a collection of 95 independent, spontaneously-occurring amber mutants affecting expression of rpoB, allows the generation of a maximum of 475 potential variants of the beta subunit of E. coli RNA polymerase, each carrying a known amino acid substitution at a particular site. The effect of these amino acid exchanges has been investigated in vivo. A significant majority (363/475) of substitutions lead to cellular death and altered properties--temperature sensitivity, apparently altered transcription termination and a changed stringent response--indicating that RNA polymerase function (unlike that of dispensable proteins) is extremely sensitive to such single site changes.

MeSH Terms
DNA-Directed RNA Polymerases/genetics Escherichia coli/enzymology,genetics Genes Macromolecular Substances Mutation Phenotype Species Specificity
Chemicals
Macromolecular Substances DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nene V
Glass R E
References (47)
47 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330040
Published
1982-00-00
Pages
399-404
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330040
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