Identification of an amber fragment of the beta subunit of Escherichia coli RNA polymerase: a yardstick for measuring controls on RNA polymerase subunit synthesis.

Molecular & general genetics : MGG ·Vol. 151 ·No. 1 ·1977-02-28 ·Pages 83-8

Glass RE

Abstract

An amber fragment of the beta subunit of Escherichia coli RNA polymerase has been recovered from strains carrying the rpoB12 amber mutation, indicating that the B12 mutation resides in the structural gene for the beta subunit. The fragment is readily assayed and can be used to determine the degree of expression of a single rpoB cistron in strains haploid or diploid for this region. These studies confirm that the bacterial mechanism, which can compensate for reduced translation of the beta message, operates by the co-ordinate induction of rpoB and rpoC. Furthermore, I show that rpo control depends upon cistron(s) located on the F' factor, KLF10, whose product(s) can act negatively in trans on rpoBC expression.

MeSH Terms

DNA-Directed RNA Polymerases/biosynthesis Escherichia coli/enzymology F Factor Genes Mutation Peptide Fragments/biosynthesis Protein Biosynthesis

Chemicals

Peptide Fragments DNA-Directed RNA Polymerases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Glass R E

References (20)

20 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00446916

Published

1977-02-28

Pages

83-8

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00446916

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Identification of an amber fragment of the beta subunit of Escherichia coli RNA polymerase: a yardstick for measuring controls on RNA polymerase subunit synthesis.

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