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PMID: 7022134 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Altered stability and integration frequency of a F' factor in RNA polymerase mutants of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 182 ·No. 1 ·1981-00-00 ·Pages 12-8

Gray GW, Chao L

Abstract

A number of spontaneous rifampicin-resistant (Rifr) mutants were isolated from a strain of E. coli having a deletion in the lac proA proB region of the chromosome. The stability of a F'lac proA proB episome in these mutants was determined by their sensitivity to acridine orange curing and the frequency of spontaneous loss of episomes. The Rifr mutants can be divided into three classes based on their ability to maintain the F'lac pro episome. Class I mutants (25% of the total Rifr mutants) showed high degree of spontaneous episome loss and high sensitivity to acridine orange curing. Class II mutants (55% of the total Rifr mutants), like the parent strains, showed intermediate sensitivity to acridine orange curing. Class III mutants (21% of the total Rifr mutants) showed high resistance to acridine orange curing and low frequency of spontaneous episome loss. Three-fourths of the Class II mutants were found to be Hfr as shown by their lack of the F'lac pro DNA band on agarose gel together with their ability to mobilize chromosomal markers in mating. Representative Rifr mutants from each class were selected and the Rifr mutants from each class were selected and the Rifr mutations were mapped within the proB gene for the beta beta' operon by P1 transduction. These results indicate that RNA polymerase, or the beta subunit of RNA polymerase, plays an important role in maintaining the F' lac pro episome and in the integration of the F' lac pro episome where no extensive sequence homology is involved.

MeSH Terms
DNA-Directed RNA Polymerases/genetics Drug Resistance, Microbial Escherichia coli/genetics,growth & development F Factor Mutation Recombination, Genetic Rifampin/pharmacology
Chemicals
DNA-Directed RNA Polymerases Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gray G W
Chao L
References (34)
34 references, click to expand
  1. Direct inhibition of Col E 1 plasmid DNA replication in Escherichia coli by rifampicin.
    Nat New Biol. 1972 May 3;237(70):29-31 PMID: 4337979
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Nucleotide-sequence analysis of Tn3 (ap): implications for insertion and deletion.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1269-77 PMID: 385227
  4. General method for the isolation of plasmid deoxyribonucleic acid.
    J Bacteriol. 1973 Nov;116(2):1064-6 PMID: 4583233
  5. Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4772-6 PMID: 337300
  6. Promoter-specific inhibition of transcription by antibiotics which act on DNA gyrase.
    Nature. 1978 Oct 5;275(5679):420-3 PMID: 211433
  7. [Mutations of the F episome of Escherichia coli K 12. II. Mutants with thermosensitive replication].
    Ann Inst Pasteur (Paris). 1967 Apr;112(4):397-418 PMID: 4865017
  8. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.
    J Bacteriol. 1976 Sep;127(3):1529-37 PMID: 821935
  9. Involvement of DNA-dependent RNA polymerase in a recA-independent pathway of genetic recombination in Escheria coli.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3932-6 PMID: 333450
  10. Chromosome replication in Escherichia coli. II. Origin of replication in F- and F+ strains.
    J Mol Biol. 1969 Oct 28;45(2):325-36 PMID: 4904407
  11. Modulation of gene expression by drugs affecting deoxyribonucleic acid gyrase.
    J Bacteriol. 1979 Apr;138(1):40-7 PMID: 108253
  12. Transcription promotes recA-independent recombination mediated by DNA-dependent RNA polymerase in Escherichia coli.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4571-5 PMID: 159459
  13. Temperature dependence of sex-factor maintenance in Escherichia coli K-12.
    J Bacteriol. 1972 Jan;109(1):447-9 PMID: 4550674
  14. Nucleotide sequence of Escherichia coli K-12 replication origin.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):575-9 PMID: 370832
  15. Electron microscopic heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F13 and related F-primes.
    J Bacteriol. 1975 May;122(2):749-63 PMID: 1092667
  16. Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome.
    J Mol Biol. 1971 Feb 14;55(3):441-56 PMID: 4927942
  17. Physiochemical studies on interactions between DNA and RNA polymerase. Ultraviolet absorption measurements.
    Nucleic Acids Res. 1978 Sep;5(9):3337-45 PMID: 360169
  18. In vivo enhancement of general and specific transcription in Escherichia coli by DNA gyrase activity.
    Gene. 1979 Oct;7(2):153-71 PMID: 229056
  19. Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):580-4 PMID: 370833
  20. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI.
    Proc Natl Acad Sci U S A. 1960 Jan;46(1):57-64 PMID: 16590598
  21. DNA gyrase and DNA supercoiling.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:35-40 PMID: 225107
  22. Local destabilization of DNA during transcription.
    J Mol Biol. 1972 Oct 28;71(1):1-9 PMID: 4564300
  23. Origin of replication, oriC, of the Escherichia coli K12 chromosome: nucleotide sequence.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:139-45 PMID: 157832
  24. Evidence for the direct involvement of RNA in the initiation of DNA replication in Escherichia coli 15T.
    J Mol Biol. 1972 Feb 28;64(1):47-60 PMID: 4552485
  25. DNA gyrase: subunit structure and ATPase activity of the purified enzyme.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):5960-3 PMID: 153529
  26. DNA gyrase and the supercoiling of DNA.
    Science. 1980 Feb 29;207(4434):953-60 PMID: 6243420
  27. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4767-71 PMID: 200930
  28. Transposition of the Lac region of E. coli.
    Cold Spring Harb Symp Quant Biol. 1966;31:393-401 PMID: 4866389
  29. DNA replication proteins of Escherichia coli.
    Annu Rev Biochem. 1978;47:1163-91 PMID: 354492
  30. Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic activity.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1773-7 PMID: 347446
  31. Chromosomal mutations affecting the stability of sex-factors in Escherichia coli.
    J Mol Biol. 1972 Jan 28;63(2):281-94 PMID: 4564179
  32. Curing of an Escherichia coli episome by rifampicin (acridine orange-F + -F - -Hfr-lac).
    Proc Natl Acad Sci U S A. 1972 Feb;69(2):298-300 PMID: 4551139
  33. Mode of action of rafamycin on the RNA polymerase reaction.
    Biochim Biophys Acta. 1968 Mar 18;157(1):218-9 PMID: 4868249
  34. A rifampicin resistent rna-polymerase from E. coli altered in the beta-subunit.
    FEBS Lett. 1969 Oct 21;5(2):104-106 PMID: 11947251
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00422760
Published
1981-00-00
Pages
12-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM26689 · United States
External Links
PubMed source DOI: 10.1007/BF00422760
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