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PMID: 358203 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Contranscription of genes for RNA polymerase subunits beta and beta' with genes for ribosomal proteins in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 8 ·1978-08-00 ·Pages 3891-5

Yamamoto M, Nomura M

Abstract

The lambdarifd18 transducing phage carries genes for RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.7.6) subunits beta and beta' (rpoB,C) and genes for four ribosomal proteins (rplK for L11, rplA for LI, rplJ for L10, and rplL for L7/L12). DNA segments of various sizes, which cover the rifd allele of the rpoB gene, were cloned into lambda vector phages. The hybrid phages were then analyzed for their ability to express the rpoB gene and neighboring ribosomal protein genes in ultraviolet-irradiated lambda-lysogenic and nonlysogenic bacterial hosts. The results show that the rpoB gene is cotranscribed with two neighboring ribosomal protein genes and that in the rpoB,C transcription unit is: promoter, rp1J, rp1L, rpoB, and rpoC.

MeSH Terms
DNA-Directed RNA Polymerases/genetics Escherichia coli/genetics Operon Ribosomal Proteins/genetics Transcription, Genetic
Chemicals
Ribosomal Proteins DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yamamoto M
Nomura M
References (28)
28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.8.3891
Published
1978-08-00
Pages
3891-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392894
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.8.3891 PMC Full Text View journal details
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