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PMID: 1101266 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

New chromosomal location for structural genes of ribosomal proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 7 ·1975-07-00 ·Pages 2765-9

Watson RJ, Parker J, Fiil NP, Flaks JG, Friesen JD

Abstract

An Escherichia coli mutant, ts9, previously reported by Flaks et al. (Cold Spring Harbor Symp. Quant. Biol. 31, 623-631, 1966) to have an electrophoretically altered ribosomal protein, has been further characterized and the altered component has been identified as L7/L12. Although mutant ts9 is temperature sensitive for growth (rts-), the rts and L7/L12 mutations are genetically separable and are both located between argH and rif. The L7/L12 mutation (rpyL) maps very close to relC, mutants of which have a defect in the 50S ribosomal subunit. The gene order is argH-rts-(rpyL,relC)-rif. Protein synthesis directed by bacteriophage lambdacI857S7drifd18 in ultraviolet-irradiated cells indicates that L7/L12, As well as L1, L10, L11, and possibly L8 or L9 are coded by the phage DNA. Our results indicate that rpyL is the structural gene for L7/L12 and that this region of the E. coli chromosome contains a cluster of structural genes for ribosomal proteins.

MeSH Terms
Bacterial Proteins/biosynthesis Chromosome Mapping Chromosomes/metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli/metabolism Genes Genotype Mutation Phenotype Protein Biosynthesis Ribosomal Proteins/biosynthesis Temperature Transcription, Genetic Transduction, Genetic
Chemicals
Bacterial Proteins Ribosomal Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Watson R J
Parker J
Fiil N P
Flaks J G
Friesen J D
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.7.2765
Published
1975-07-00
Pages
2765-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432852
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.7.2765 PMC Full Text View journal details
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