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PMID: 1090938 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Specialized transducing phages for ribosomal protein genes of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 1 ·1975-01-00 ·Pages 6-10

Jaskunas SR, Lindahl L, Nomura M

Abstract

Specialized lambda transducing phages have been isolated carrying approximately half the ribosomal protein genes of E. coli. These phages carry regions of the bacterial chromosome between aroE and fus. The ribosomal protein genes on these phages have been identified by the stimulation of ribosomal protein synthesis in ultraviolet-irradiated bacteria following infection by the transducing phage, and by the in vitro synthesis of ribosomal proteins in a DNA-dependent protein synthesizing system. The results indicate lambdadspcl probably carries at least 22 ribosomal protein genes and lambdadspc2 at least 26 genes. All these genes are clustered between trkA and strA. At least 13 of them have not been previously mapped.

MeSH Terms
Bacterial Proteins/biosynthesis Carbon Radioisotopes Chromosome Mapping Chromosomes, Bacterial/metabolism Coliphages/metabolism DNA Viruses/metabolism DNA, Bacterial/metabolism DNA, Viral/metabolism Escherichia coli/metabolism Genes Leucine/metabolism Methionine/metabolism Radioimmunoassay Ribosomes/metabolism Sulfur Radioisotopes Transduction, Genetic Tritium
Chemicals
Bacterial Proteins Carbon Radioisotopes DNA, Bacterial DNA, Viral Sulfur Radioisotopes Tritium Methionine Leucine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jaskunas S R
Lindahl L
Nomura M
References (17)
17 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.1.6
Published
1975-01-00
Pages
6-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432228
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.1.6 PMC Full Text View journal details
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