Home LiteratureArticle Details
PMID: 6353162 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

An amber replication mutant of F plasmid mapped in the minimal replication region.

Molecular & general genetics : MGG ·Vol. 191 ·No. 2 ·1983-00-00 ·Pages 231-7

Maki S, Kuribayashi M, Miki T, Horiuchi T

Abstract

We have constructed a mini-F derivative (pKP1013) consisting of a 5.4 kilobase pairs (kb) segment (44.0 to 49.4 kb) of mini-F and fragments carrying the chloramphenicol and spectinomycin resistance genes that originated from the R plasmid NR1. The plasmid pKP1013 replicates autonomously in a manner indistinguishable from that of the parental mini-F. An amber mutant defective in replication has been isolated from pKP1013 by localized mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine. The virtual absence of incorporation of [3H]-thymidine into the plasmid DNA as well as the kinetics of appearance of plasmid-free segregants suggest that plasmid DNA synthesis is primarily affected under nonpermissive conditions. The amber mutation has been mapped within the 530 base pairs (bp) region that extends from 45.25 (XmaI) to 45.78 Kb (PstI) by extensive analysis of in vitro recombinants constructed from rep+ and rep- plasmids.

MeSH Terms
Chromosome Mapping DNA Replication DNA, Bacterial/biosynthesis Escherichia coli/genetics,metabolism F Factor Genes, Bacterial Mutation
Chemicals
DNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Maki S
Kuribayashi M
Miki T
Horiuchi T
References (25)
25 references, click to expand
  1. The number of sex-factors per chromosome in Escherichia coli.
    Biochem J. 1971 Jan;121(1):93-103 PMID: 4940379
  2. Temperature-sensitive mutations affecting the replication of F-prime factors in Escherichia coli K 12.
    Mol Gen Genet. 1973 Dec 31;127(4):297-306 PMID: 4594002
  3. Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid.
    Gene. 1981 Nov;15(2-3):257-71 PMID: 7028572
  4. Mutants of Escherichia coli requiring methionine or vitamin B12.
    J Bacteriol. 1950 Jul;60(1):17-28 PMID: 15436457
  5. Genetic studies of F plasmid maintenance genes involved in copy number control, incompatability, and partitioning.
    Plasmid. 1982 Mar;7(2):163-79 PMID: 6281833
  6. [Mutations of the F episome of Escherichia coli K 12. II. Mutants with thermosensitive replication].
    Ann Inst Pasteur (Paris). 1967 Apr;112(4):397-418 PMID: 4865017
  7. Mapping of the resistance genes of the R plasmid NR1.
    Mol Gen Genet. 1978 Jan 17;158(3):217-24 PMID: 146819
  8. Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid.
    J Bacteriol. 1976 Aug;127(2):988-97 PMID: 783125
  9. Cloning, isolation, and characterization of replication regions of complex plasmid genomes.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2242-6 PMID: 1094460
  10. Essential regions for replication of a stringent and a relaxed plasmid in Escherichia coli.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:99-103 PMID: 383389
  11. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
    J Bacteriol. 1974 Sep;119(3):1072-4 PMID: 4605400
  12. Replication mutants of the F-plasmid of Escherichia coli. II. Cloned replication regions of temperature-sensitive mutants.
    Plasmid. 1980 Mar;3(2):179-92 PMID: 6765557
  13. Strand and site specificity of the relaxation event for the relaxation complex of the antibiotic resistance plasmid R6K.
    Biochemistry. 1974 Dec 31;13(27):5484-90 PMID: 4616719
  14. Indirect selection for plasmid mutants: isolation of ColVBtrp mutants defective in self-maintenance in Escherichia coli.
    J Bacteriol. 1975 Apr;122(1):73-9 PMID: 1091632
  15. Cell length, cell growth and cell division.
    Nature. 1976 Nov 25;264(5584):328-33 PMID: 794735
  16. Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid.
    J Bacteriol. 1976 Aug;127(2):982-7 PMID: 783124
  17. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  18. Direct repeats of the F plasmid incC region express F incompatibility.
    Cell. 1981 Jun;24(3):687-94 PMID: 7018695
  19. Amber-mutants of plasmid mini-F defective in replication.
    Mol Gen Genet. 1980;180(1):201-4 PMID: 7003308
  20. Cloning of replication, incompatibility, and stability functions of R plasmid NR1.
    J Bacteriol. 1980 Jan;141(1):87-99 PMID: 6243631
  21. Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments.
    Mol Gen Genet. 1982;186(2):193-203 PMID: 7050629
  22. Bidirection replication from a unique origin in a mini-F plasmid.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1138-41 PMID: 322144
  23. Induction of closely linked multiple mutations by nitrosoguanidine.
    Nat New Biol. 1971 Mar 24;230(12):122-5 PMID: 4927375
  24. Analysis of mini-F plasmid replication by transposition mutagenesis.
    J Bacteriol. 1981 Sep;147(3):888-99 PMID: 6268613
  25. Mutants of the mini-F plasmid pML31 thermosensitive in replication.
    J Bacteriol. 1979 May;138(2):559-66 PMID: 374396
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334819
Published
1983-00-00
Pages
231-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00334819
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2