Indirect selection for plasmid mutants: isolation of ColVBtrp mutants defective in self-maintenance in Escherichia coli.
An efficient method for isolation of a large number of plasmid mutants is described. It is based on the fact that N-methyl-N'-nitro-N-nitrosoguanidine induces a number of closely linked mutations within a short segment of the bacterial chromosome. Thus, selection for reversions of an auxotrophic marker located on the ColVBtrp plasmid yielded a large fraction (more than 50 percent) of mutants defective in some plasmid functions, including its own maintenance in the host bacteria. The results of preliminary characterization of strains carrying these mutated plasmids are presented.
13 references, click to expand
-
Acetylornithinase of Escherichia coli: partial purification and some properties.J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
-
The properties of altered proteins from mutants bearing one or two lesions in the same gene.Proc Natl Acad Sci U S A. 1961 Apr 15;47:551-66 PMID: 13765795
-
Plasmid mutations affecting self-maintenance and host growth in Escherichia coli.J Bacteriol. 1975 Apr;122(1):80-8 PMID: 804472
-
Induction of closely linked multiple mutations by nitrosoguanidine.Nat New Biol. 1971 Mar 24;230(12):122-5 PMID: 4927375
-
On the association between DNA and membrane in bacteria.Proc R Soc Lond B Biol Sci. 1966 Mar 22;164(995):267-78 PMID: 4379516
-
Molecular structure of bacterial plasmids.Bacteriol Rev. 1972 Sep;36(3):361-405 PMID: 4345849
-
Transcription of the tryptophan operon of Escherichia coli in vitro. I. Detection and quantitative determination of specific RNA.Biochim Biophys Acta. 1968 Dec 17;169(2):494-510 PMID: 4302870
-
Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation.J Bacteriol. 1972 Jun;110(3):843-51 PMID: 4555413
-
Regulation of chromosome replication and the bacterial cell cycle.Annu Rev Microbiol. 1972;26:347-68 PMID: 4562814
-
Cosegregation of a sex factor with the Escherichia coli chromosome during curing by acridine orange.J Mol Biol. 1969 Oct 28;45(2):385-95 PMID: 4904409
-
The replication of the Escherichia coli chromosome studied by sequential nitrosoguanidine mutagenesis.Cold Spring Harb Symp Quant Biol. 1968;33:599-607 PMID: 4891996
-
Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants.J Bacteriol. 1971 May;106(2):529-38 PMID: 4929865
-
Circular DNA forms of a bacterial sex factor.Proc Natl Acad Sci U S A. 1967 Oct;58(4):1731-8 PMID: 5237898
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
