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PMID: 783125 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid.

Journal of bacteriology ·Vol. 127 ·No. 2 ·1976-08-00 ·Pages 988-97

Guyer MS, Figurski D, Davidson N

Abstract

pML31, a plasmid chimera constructed to contain the replication genes of an Flac plasmid, has been studied by electron microscope methods. Heteroduplex analysis shows that the only F sequence present in pML31 is that with corrdinates 40.3-49.3F. This region has previously been identified as essential for plasmid maintenance. The sequence of pML31, which was derived originally from R6-5, carries the km gene(s) and an inverted duplication of a 1.0-kilobase sequence. On the basis of length measurements, the repeated sequence is different from IS1, IS2, IS3, and an inverted repeat associated with the km gene(s) of plasmid JR67.

MeSH Terms
Base Sequence Conjugation, Genetic DNA Replication DNA, Bacterial/analysis,biosynthesis DNA, Circular/analysis DNA, Single-Stranded/analysis Escherichia coli/analysis,metabolism F Factor Genes Microscopy, Electron Models, Structural
Chemicals
DNA, Bacterial DNA, Circular DNA, Single-Stranded
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Guyer M S
Figurski D
Davidson N
References (38)
38 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.127.2.988-997.1976
Published
1976-08-00
Pages
988-97
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233009
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.127.2.988-997.1976 PMC Full Text View journal details
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