Home LiteratureArticle Details
PMID: 374396 Published · ppublish English Journal Article

Mutants of the mini-F plasmid pML31 thermosensitive in replication.

Journal of bacteriology ·Vol. 138 ·No. 2 ·1979-05-00 ·Pages 559-66

Eichenlaub R

Abstract

Hydroxylamine mutagenesis was used for the induction of thermosensitive replication mutants of the mini-F plasmid pML31. Replication mutants were characterized by studying the segregation kinetics and the incorporation of [3H]-thymidine into plasmid deoxyribonucleic acid at the nonpermissive temperature. Based on these experiments two types of mutants could be distinguished. Mutants of type I are fast segregating with the kinetics expected if plasmid replication was blocked immediately. Double-label experiments showed a rapid shut-off of replication in these mutants at 42 degrees C. Mutants of type II segregate slower, showing only a partial inhibition of plasmid deoxyribonucleic acid synthesis at the nonpermissive temperature. The label incorporated at 42 degrees C was predominantly found in open circular plasmid molecules.

MeSH Terms
DNA Replication DNA, Bacterial/biosynthesis DNA, Circular/biosynthesis DNA, Superhelical/biosynthesis Escherichia coli/genetics,metabolism Hydroxylamines Mutagens Mutation R Factors Temperature
Chemicals
DNA, Bacterial DNA, Circular DNA, Superhelical Hydroxylamines Mutagens
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Eichenlaub R
References (24)
24 references, click to expand
  1. Temperature-dependent and amber copy mutants of plasmid R1drd-19 in Escherichia coli.
    Plasmid. 1978 Feb;1(2):134-44 PMID: 372959
  2. Altered DNA-protein relaxation complex in a replication mutant of plasmid ColE1.
    Mol Gen Genet. 1978 Nov 16;167(1):21-8 PMID: 368585
  3. Mutations of temperature sensitivity in R plasmid pSC101.
    J Bacteriol. 1977 Aug;131(2):405-12 PMID: 328479
  4. Replication of thermosensitive Rts1 plasmid deoxyribonucleic acid at the nonpermissive temperature.
    J Bacteriol. 1977 Oct;132(1):90-9 PMID: 334735
  5. Bidirection replication from a unique origin in a mini-F plasmid.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1138-41 PMID: 322144
  6. Role of dnaB43 in F'-plasmid incompatibility.
    Mol Gen Genet. 1977 Jan 18;150(2):161-70 PMID: 320452
  7. Re-examination of F plasmid replication in a dnaC mutant of Escherichia coli.
    Mol Gen Genet. 1977 Feb 15;150(3):285-92 PMID: 321937
  8. Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.
    J Bacteriol. 1973 May;114(2):577-91 PMID: 4350343
  9. Description of an incompatibility mutant of Escherichia coli.
    J Bacteriol. 1973 Jul;115(1):213-20 PMID: 4577742
  10. General method for the isolation of plasmid deoxyribonucleic acid.
    J Bacteriol. 1973 Nov;116(2):1064-6 PMID: 4583233
  11. DNA polymerase 3 and the replication of F and ColVBtrp in Escherichia coli K-12.
    Mol Gen Genet. 1973 Dec 31;127(3):255-8 PMID: 4593027
  12. Replication of plasmid DNA in temperature-sensitivity DNA replication mutants of Escherichia coli.
    Biochim Biophys Acta. 1972 Feb 23;262(1):32-41 PMID: 4552902
  13. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  14. Replication of the F'lac sex factor in the cell cycle of Escherichia coli.
    Mol Gen Genet. 1971;111(3):242-55 PMID: 4935354
  15. Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome.
    J Mol Biol. 1971 Feb 14;55(3):441-56 PMID: 4927942
  16. Inhibition of replication of an F'lac episome in Hfr cells of Escherichia coli.
    J Bacteriol. 1968 Feb;95(2):531-9 PMID: 4867745
  17. [Mutations of the F episome of Escherichia coli K 12. II. Mutants with thermosensitive replication].
    Ann Inst Pasteur (Paris). 1967 Apr;112(4):397-418 PMID: 4865017
  18. Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid.
    J Bacteriol. 1976 Aug;127(2):988-97 PMID: 783125
  19. Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid.
    J Bacteriol. 1976 Aug;127(2):982-7 PMID: 783124
  20. Cloning, isolation, and characterization of replication regions of complex plasmid genomes.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2242-6 PMID: 1094460
  21. Isolation and characterization of Escherichia coli chromosomal mutants affecting plasmid copy number.
    J Bacteriol. 1976 Feb;125(2):635-42 PMID: 1107327
  22. Initiation of DNA replication in Escherichia coli. II. Effect of rifampicin on the resumption of replication of F episome and chromosome upon the returning of dna mutants from a non-permissive to a permissive temperature.
    Mol Gen Genet. 1975;137(3):239-48 PMID: 1102916
  23. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons.
    Nature. 1976 Jan 29;259(5541):285-90 PMID: 765836
  24. Mutagenesis of plasmid DNA with hydroxylamine: isolation of mutants of multi-copy plasmids.
    Mol Gen Genet. 1976 Apr 23;145(1):101-8 PMID: 775304
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.138.2.559-566.1979
Published
1979-05-00
Pages
559-66
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218213
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.138.2.559-566.1979 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2