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PMID: 320452 Published · ppublish English Journal Article

Role of dnaB43 in F'-plasmid incompatibility.

Molecular & general genetics : MGG ·Vol. 150 ·No. 2 ·1977-01-18 ·Pages 161-70

Jamieson AF, Bergquist PL

Abstract

In order to perform complementation tests with mutations of DNA replication of F'-plasmids incompatibility must overcome. We report out in ability to duplicate the results presented by Palchoudhury and Iyer (1971) and Bezanson and Iyer (1975) who have claimed to demonstrate the autonomous replication two incompatible F'-plasmids in a strain carrying the temperature sensitive dna B43 allele. In addition, we describe experiments designed to measured complementation using transient heterozygotes and compatible plasmids. Assessment of our data and those of others in the light of a recent report by Uhlin and Nordström (1975) suggests that new approaches will have to be developed for the successful employment of complementation analysis in F-plasmid genetics.

MeSH Terms
Conjugation, Genetic DNA Replication Escherichia coli/metabolism F Factor Genetic Complementation Test Heterozygote Hot Temperature Mutation Transduction, Genetic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jamieson A F
Bergquist P L
References (25)
25 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
Published
1977-01-18
Pages
161-70
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
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