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PMID: 334735 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Replication of thermosensitive Rts1 plasmid deoxyribonucleic acid at the nonpermissive temperature.

Journal of bacteriology ·Vol. 132 ·No. 1 ·1977-10-00 ·Pages 90-9

Yamamoto T, Kaji A

Abstract

Replication of the thermosensitive drug resistance factor Rts1 was studied at the nonpermissive temperature (42 degrees C). It was concluded from the following observations that replication of this plasmid takes place at 42 degrees C without involving the covalently closed circular (CCC) form of deoxyribonucleic acid (DNA). (i) DNA-DNA- reassociation kinetics studies with purified Rts1 DNA showed that Rts1 DNA increased several-fold during cell growth at 42 degrees C while very little, if any, CCC DNA was synthesized. (ii) When Escherichia coli 20S0(Rts1) was labeled with [3H]thymidine at 42 degrees C, a significant amount of radioactive DNA hybridizable to Rts1 DNA was formed. This DNA was found in a fraction where DNA other than CCC DNA was expected in alkaline sucrose density gradient centrifugation analysis. When E. coli 20S0(Rts1) was labeled at 32 degrees C, the labeled CCC DNA did not disappear during a chase period at 42 degrees C. This indicates that preformed CCC DNA does not participate in replication at the nonpermissive temperature. These results are consistent with the hypothesis that there are two modes of replication of Rts1 DNA, one involving a CCC molecule and the other not involving this form, and that only the latter mode takes place at the nonpermissive temperature.

MeSH Terms
DNA Replication DNA, Bacterial/biosynthesis,genetics DNA, Circular/biosynthesis Escherichia coli/drug effects,genetics,metabolism Kanamycin/pharmacology Mutation R Factors Temperature
Chemicals
DNA, Bacterial DNA, Circular Kanamycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yamamoto T
Kaji A
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41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.132.1.90-99.1977
Published
1977-10-00
Pages
90-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221830
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.132.1.90-99.1977 PMC Full Text View journal details
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