The thermosensitive lesion in the replication of the drug resistance factor, Rts1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 6 ·1974-06-00 ·Pages 2515-9

DiJoseph CG

Abstract

The DNA of the thermosensitive R factor, Rts1, has been examined by the technique of sedimentation in alkaline sucrose density gradients. Rts1 DNA was found as closed covalent circles in only a few copies per cell in an Escherichia coli host at the permissive temperature. Rts1 DNA appears to be synthesized at the nonpermissive temperature, but was not found as closed covalent circles. However, circular DNA could be recovered upon shift down to the permissive temperature. The large number of plasmid-negative cells which accumulate after prolonged culture at non-permissive temperature may be due to a strong selective pressure favoring the growth of rare R(-) segregants.

MeSH Terms

Centrifugation, Density Gradient DNA Replication DNA, Bacterial/isolation & purification DNA, Circular/isolation & purification Escherichia coli/analysis,metabolism Extrachromosomal Inheritance Kanamycin Molecular Weight Penicillin Resistance Penicillins Temperature

Chemicals

DNA, Bacterial DNA, Circular Penicillins Kanamycin

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

DiJoseph C G

References (22)

22 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.6.2515

Published

1974-06-00

Pages

2515-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388490

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.6.2515 PMC Full Text View journal details

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The thermosensitive lesion in the replication of the drug resistance factor, Rts1.

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