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PMID: 7050629 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments.

Molecular & general genetics : MGG ·Vol. 186 ·No. 2 ·1982-00-00 ·Pages 193-203

Komai N, Nishizawa T, Hayakawa Y, Murotsu T, Matsubara K

Abstract

Various DNA subfragments were derived from miniF DNA by complete or partial PstI cleavage, and cloned in the plasmid vectors pBR322 or lambda dv1. The recombinant plasmids obtained were introduced into an Escherichia coli minicell-producing strain, and the plasmid-coded proteins were radiolabeled and analyzed by gel electrophoresis. Six miniF-encoded proteins, larger than 11 000 daltons, were detected and their coding regions were mapped on the F plasmid genome. Three of them were assigned by taking into account the known nucleotide sequences (Murotsu et al. 1981; K. Yoshioka, personal communication). The coding directions of some proteins were determined by inserting the lac promotor into one of the recombinant plasmids and analyzing the increase in production of the proteins. The coding direction of the five proteins analyzed so far was uniform. Comparison of these results with a functional map of miniF suggested possible roles of the proteins.

MeSH Terms
Bacterial Proteins/genetics Chromosome Mapping DNA Replication DNA, Bacterial/genetics DNA, Recombinant Escherichia coli/genetics F Factor Genes Genes, Bacterial Replicon
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Recombinant
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Komai N
Nishizawa T
Hayakawa Y
Murotsu T
Matsubara K
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44 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331850
Published
1982-00-00
Pages
193-203
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00331850
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