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PMID: 6268604 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complementation of replication-deficient deletion derivatives of plasmid mini-F.

Journal of bacteriology ·Vol. 147 ·No. 3 ·1981-09-00 ·Pages 736-43

Ebbers J, Eichenlaub R

Abstract

Deleted mini-F plasmids with defects in replication were constructed and tested to see whether they could be rescued through complementation by a helper plasmid. This allowed us to identify two genetic loci determining trans-acting functions required for stable maintenance of plasmid mini-F, one encoded by the PstI fragment from 45.7 to 47.3 F-coordinates (F) and the other most probably located in the region from 43.1 to 43.8 F. The smallest mini-F plasmid that could be established through complementation consists of the PstI fragment 44.0 to 45.7 F, encoding origin II and the incB locus.

MeSH Terms
DNA Replication DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific Escherichia coli/genetics F Factor Genetic Complementation Test Plasmids Transformation, Bacterial
Chemicals
DNA Restriction Enzymes CTGCAG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ebbers J
Eichenlaub R
References (31)
31 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.147.3.736-743.1981
Published
1981-09-00
Pages
736-43
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216108
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.147.3.736-743.1981 PMC Full Text View journal details
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