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PMID: 16094996 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication.

Molecular & general genetics : MGG ·Vol. 136 ·No. 4 ·1975-00-00 ·Pages 273-89

Collins J, Williams P, Helinski DR

Abstract

Mutants of the dnaA, dnaC, dnaD, polC, dnaF and dnaG gene loci were tested for their capacity for colicinogenic plasmid E1 (ColE1) replication at a non-permissive temperature. It was found that ColE1 replication was independent of the dnaA gene function and dependent on dnaC, D, F and G. ColE1 replication in the polC mutant E486 continued for several hours but at a greatly reduced rate. No effect was found of the dnaG mutation on thymine-deprivation-induced "priming" of ColE1 replication at the non-permissive temperature. The mutants also were tested for aberrant replication intermediates of plasmid DNA as well as a temperature sensitive supercoiled DNA-protein relaxation complex. RNA-containing supercoils were found to accumulate in a poIC mutant also blocked for protein synthesis.

MeSH Terms
DNA Replication DNA, Bacterial/genetics DNA, Superhelical/genetics Escherichia coli/genetics Mutation Plasmids Temperature
Chemicals
DNA, Bacterial DNA, Superhelical
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Collins J
Department of Biology, University of California, San Diego, La Jolla, California 92037, USA.
Williams P
Helinski D R
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30 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00341713
Published
1975-00-00
Pages
273-89
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIAID NIH HHS · AI-07194 · United States
External Links
PubMed source DOI: 10.1007/BF00341713
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