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PMID: 146819 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mapping of the resistance genes of the R plasmid NR1.

Molecular & general genetics : MGG ·Vol. 158 ·No. 3 ·1978-01-17 ·Pages 217-24

Miki T, Easton AM, Rownd RH

Abstract

The drug resistance genes on the r-determinants component of the composite R plasmid NR1 were mapped on the EcoRI restriction endonuclease fragments of the R plasmid by cloning the fragments using the plasmid RSF2124 as a vector. The sulfonamide (Su) and streptomycin/spectinomycin (Sm/Sp) resistance genes are located on EcoRI fragment G of NR1. The expression of resistance to mercuric ions (Mer) requires both EcoRI fragment H and I of NR1. The expression of chloramphenicol (Cm) and fusidic acid (Fus) resistance requires EcoRI fragments A and J of NR1. The kan fragment of the related R plasmid R6-5 can substitute for Eco RI fragment J of NR1 in the expression of Cm and Fus resistance. The structural genes for Cm and Fus resistance appear to be a part of an operon whose expression is controlled by the same promoter.

MeSH Terms
Chloramphenicol/pharmacology Escherichia coli/genetics Fusidic Acid/pharmacology Genes Genetic Linkage Mercury/pharmacology R Factors Spectinomycin/pharmacology Streptomycin/pharmacology Sulfonamides/pharmacology Tetracycline/pharmacology
Chemicals
Sulfonamides Fusidic Acid Chloramphenicol Spectinomycin Tetracycline Mercury Streptomycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miki T
Easton A M
Rownd R H
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29 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267192
Published
1978-01-17
Pages
217-24
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267192
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