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PMID: 6346058 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 3 ·No. 5 ·1983-05-00 ·Pages 839-53

Bostian KA, Lemire JM, Halvorson HO

Abstract

We have examined the regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum], EC 3.1.3.2) in Saccharomyces cerevisiae at the physiological and molecular levels, through a series of repression and derepression experiments. We demonstrated that APase synthesis is tightly regulated throughout the growth phase and is influenced by exogenous and endogenous Pi pools. During growth in a nonlimiting Pi medium, APase is repressed. When external Pi becomes limiting, there is a biphasic appearance of APase mRNA and enzyme. Our data on APase mRNA half-lives and on the flux of intracellular Pi and polyphosphate during derepression are consistent with a mechanism of transcriptional autoregulation for the biphasic appearance of APase mRNA. Accordingly, preculture concentrations of Pi control the level of corepressor generated from intracellular polyphosphate degradation. When cells are fully derepressed, APase mRNA levels are constant, and the maximal linear accumulation rate of APase is observed. A scheme to integrate phosphorus metabolism and phosphatase regulation in S. cerevisiae is proposed.

MeSH Terms
Acid Phosphatase/genetics Enzyme Repression Feedback Phosphates/metabolism RNA, Messenger/biosynthesis RNA, Viral/biosynthesis Saccharomyces cerevisiae/enzymology,genetics Viral Proteins/genetics
Chemicals
Phosphates RNA, Messenger RNA, Viral Viral Proteins Acid Phosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bostian K A
Lemire J M
Halvorson H O
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45 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.3.5.839-853.1983
Published
1983-05-00
Pages
839-53
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368607
Subset
IM
Grants
PHS HHS · AL-10610 · United States
External Links
PubMed source DOI: 10.1128/mcb.3.5.839-853.1983 PMC Full Text View journal details
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