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PMID: 226972 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Transcriptional regulation of the yeast cytochrome c gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 8 ·1979-08-00 ·Pages 3627-31

Zitomer RS, Montgomery DL, Nichols DL, Hall BD

Abstract

DNA from the cloned yeast iso-1-cytochrome c, cycl, gene was used in a hybridization assay to measure levels and rates of synthesis of cycl RNA. Derepressed cells synthesized cycl RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcription of the cycl gene was repressed within 2.5 min. The half-life of hybridizable cycl RNA was determined to be 12-13.5 min under repressed and derepressed conditions and during repression. The results demonstrate that the expression of the cycl gene is subject to transcriptional regulation.

MeSH Terms

Cytochrome c Group/genetics DNA, Recombinant Genes Glucose/metabolism Nucleic Acid Hybridization RNA, Messenger/metabolism Raffinose/metabolism Saccharomyces cerevisiae/genetics Transcription, Genetic

Chemicals

Cytochrome c Group DNA, Recombinant RNA, Messenger Glucose Raffinose

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Zitomer R S

Montgomery D L

Nichols D L

Hall B D

References (12)

12 references, click to expand

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Deletions of the iso-1-cytochrome c and adjacent genes of yeast: discovery of the OSM1 gene controlling osmotic sensitivity.
Genetics. 1978 Aug;89(4):653-65 PMID: 211068
Identification and isolation of the yeast cytochrome c gene.
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Kinetics of glucose repression of yeast cytochrome c.
J Bacteriol. 1978 Jul;135(1):39-44 PMID: 209012
Yeast cytochrome c messenger RNA. In vitro translation and specific immunoprecipitation of the CYC1 gene product.
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Evidence for transcriptional regulation of orotidine-5'-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli.
Proc Natl Acad Sci U S A. 1979 Jan;76(1):386-90 PMID: 370827
Mutants of yeast defective in iso-1-cytochrome c.
Genetics. 1974 Jun;77(2):255-84 PMID: 4367877
Decay of messenger ribonucleic acid from the lactose operon of Escherichia coli as a function of growth temperature.
J Mol Biol. 1973 Feb 15;74(1):21-31 PMID: 4581286
Biogenesis of mRNA: genetic regulation in mammalian cells.
Science. 1973 Sep 28;181(4106):1215-21 PMID: 4542191
Difference between functional and structural integrity of messenger RNA.
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2171-5 PMID: 4516212
Plasmid-linked ampicillin resistance in haempohilus influenza type b.
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Genetic determination of iso-cytochromes c in yeast.
J Mol Biol. 1965 Aug;13(1):21-39 PMID: 5859036

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.76.8.3627

Published

1979-08-00

Pages

3627-31

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC383885

Subset

External Links

PubMed source DOI: 10.1073/pnas.76.8.3627 PMC Full Text View journal details

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