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PMID: 6212932 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Acid phosphatase polypeptides in Saccharomyces cerevisiae are encoded by a differentially regulated multigene family.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 7 ·1982-04-00 ·Pages 2157-61

Rogers DT, Lemire JM, Bostian KA

Abstract

Two clones from a lambda phage collection containing yeast genes regulated by inorganic phosphate were shown by low-stringency hybridization to select three mRNAs that direct the in vitro synthesis of repressible acid phosphatase (EC 3.1.3.2) polypeptides p60, p58, and p56. By higher stringency hybridization one yeast fragment [8 kilobases (kb)] selects p60 mRNA and the other (5 kb) selects p56 mRNA. These EcoRI digestion fragments were subcloned in yeast transformation vectors and hybridization selection assignments were confirmed by measuring enzyme and mRNA levels in transformants. Enzyme and mRNA levels in (8-kb) high copy number transformants grown in high inorganic phosphate medium revealed a hitherto undetected acid phosphatase protein, P57, which is believed to correspond to the constitutive enzyme encoded by PHO3. The identify of the 8-kb fragment purported to contain the PHO5/PHO3 genes was confirmed by genetic mapping of an integrated copy of this fragment. The site of integration of the 5-kb fragment was demonstrated to be unlinked to the PHO5/PHO3 genes.

MeSH Terms
Acid Phosphatase/genetics Bacteriophage lambda/genetics Chromosome Mapping DNA, Recombinant/metabolism Genes Nucleic Acid Hybridization Operon RNA, Messenger/genetics,metabolism Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
DNA, Recombinant RNA, Messenger Acid Phosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rogers D T
Lemire J M
Bostian K A
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.7.2157
Published
1982-04-00
Pages
2157-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346149
Subset
IM
Grants
NIAID NIH HHS · AI10610 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.7.2157 PMC Full Text View journal details
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