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PMID: 6256743 Published · ppublish English Journal Article

Isolation of yeast genes with mRNA levels controlled by phosphate concentration.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 11 ·1980-11-00 ·Pages 6541-5

Kramer RA, Andersen N

Abstract

A library of DNA from the yeast, Saccharomyces cerevisiae, was constructed in phage lambda Charon 4 vector and then screened by differential plaque filter hybridization for genes induced by phosphate starvation. Two EcoRI fragments of 7.9 and 5.0 kilobase pairs that contained such genes were isolated. These cloned fragments may each carry one of the several copies of the genes for the repressible acid phosphatase of yeast. The fragments were use to examine mRNA levels of these genes in regulatory mutants of acid phosphatase.

MeSH Terms
Gene Expression Regulation Genes Genes, Regulator Phosphates/physiology Phosphoric Monoester Hydrolases/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Phosphates RNA, Messenger Phosphoric Monoester Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kramer R A
Andersen N
References (22)
22 references, click to expand
  1. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  2. Regulation and characterization of acid and alkaline phosphatase in yeast.
    J Gen Microbiol. 1971 Mar;65(3):291-303 PMID: 4997392
  3. Isolation and characterization of acid phosphatase mutants in Saccharomyces cerevisiae.
    J Bacteriol. 1973 Feb;113(2):727-38 PMID: 4570606
  4. Isolation and characterization of recessive, constitutive mutations for repressible acid phosphatase synthesis in Saccharomyces cerevisiae.
    J Bacteriol. 1975 Jun;122(3):911-22 PMID: 1097406
  5. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  6. Genes coding for the structure of the acid phosphatases in Saccharomyces cerevisiae.
    Mol Gen Genet. 1975 Dec 30;143(1):65-70 PMID: 765744
  7. A gene controlling the synthesis of non specific alkaline phosphatase in Saccharomyces cerevisiae.
    Biochim Biophys Acta. 1976 Mar 25;428(1):182-92 PMID: 769832
  8. Hybridization of RNA to double-stranded DNA: formation of R-loops.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2294-8 PMID: 781674
  9. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  10. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  11. Analysis of chromosomal integration and deletions of yeast plasmids.
    Nucleic Acids Res. 1977;4(5):1429-48 PMID: 331256
  12. A comparison of two cloned mouse beta-globin genes and their surrounding and intervening sequences.
    Cell. 1978 Jun;14(2):237-45 PMID: 566620
  13. Disturbance of the machinery for the gene expression by acidic pH in the repressible acid phosphatase system of Saccharomyces cerevisiae.
    Mol Gen Genet. 1978 Jun 14;162(2):139-49 PMID: 27717
  14. Isolation, characterization, and translation of mRNA from yeast.
    Methods Cell Biol. 1978;20:61-81 PMID: 357927
  15. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  16. Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridization.
    Cell. 1979 Feb;16(2):443-52 PMID: 378392
  17. Posttranslational regulation of repressible acid phosphatase in yeast.
    Mol Gen Genet. 1979 Jun 20;173(3):349-51 PMID: 384156
  18. Interference of nonsense mutations with eukaryotic messenger RNA stability.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5134-7 PMID: 388431
  19. Restriction maps for twenty-one Charon vector phages.
    J Virol. 1980 Jan;33(1):401-10 PMID: 6245241
  20. In vitro packaging of lambda Dam vectors and their use in cloning DNA fragments.
    Methods Enzymol. 1979;68:281-98 PMID: 232217
  21. Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1437-41 PMID: 6445556
  22. In vitro synthesis of repressible yeast acid phosphatase: identification of multiple mRNAs and products.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4504-8 PMID: 7001459
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.11.6541
Published
1980-11-00
Pages
6541-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350321
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.11.6541 PMC Full Text View journal details
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