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PMID: 9279376 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcriptional regulation of the Kluyveromyces lactis beta-galactosidase gene.

Molecular and cellular biology ·Vol. 1 ·No. 7 ·1981-07-00 ·Pages 629-34

Lacy LR, Dickson RC

Abstract

We examined the molecular basis for beta-D-galactosidase (EC 3.2.1.23) induction in the yeast Kluyveromyces lactis. The protein synthesis inhibitor anisomycin effectively blocked both protein synthesis and enzyme induction by lactose. Further, hybridization analysis with the cloned beta-galactosidase gene indicated coordinate increases in the concentration of beta-galactosidase messenger ribonucleic acid and enzyme activity. The half-life of beta-galactosidase messenger ribonucleic acid was the same (4.8 +/- 0.4 min) when measured both before and at succeeding times during enzyme induction. These results strongly support the hypothesis that expression of the yeast beta-galactosidase gene is subject to transcriptional regulation.

MeSH Terms
Anisomycin/pharmacology Enzyme Induction Half-Life Kluyveromyces/enzymology,genetics Lactose/pharmacology Protein Synthesis Inhibitors/pharmacology RNA, Messenger/metabolism Transcription, Genetic beta-Galactosidase/biosynthesis,genetics
Chemicals
Protein Synthesis Inhibitors RNA, Messenger Anisomycin beta-Galactosidase Lactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lacy L R
Department of Biochemistry, University of Kentucky, Lexington 40536, USA.
Dickson R C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.1.7.629-634.1981
Published
1981-07-00
Pages
629-34
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369710
Subset
IM
Grants
NIGMS NIH HHS · GM-22749 · United States
NCRR NIH HHS · RR0537415 · United States
External Links
PubMed source DOI: 10.1128/mcb.1.7.629-634.1981 PMC Full Text View journal details
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