Interaction of cations with phosphate uptake by Saccharomyces cerevisiae. Effects of surface potential.

The Biochemical journal ·Vol. 178 ·No. 3 ·1979-03-15 ·Pages 521-7

Roomans GM, Borst-Pauwels GW

Abstract

The effect of bivalent cations on phosphate uptake by Saccharomyces cerevisiae was investigated. Phosphate uptake via the Na+-dependent transport system at pH 7.2 is stimulated by bivalent cations. The apparent affinity of phosphate for the transport mechanism is increased, but the apparent affinity for Na+ is decreased. Uptake of phosphate via the Na+-independent transport system is accompanied by a net proton influx of 2H+ and an efflux of 1 K+ for each phosphate ion taken up. At pH 4.5 phosphate uptake via the Na+-independent system is stimulated by bivalent cations, whereas at pH 7.2 uptake is inhibited. The effect of bivalent cations on phosphate uptake can be ascribed to a decrease in the surface potential.

MeSH Terms

Binding Sites Biological Transport/drug effects Calcium/pharmacology Electrophysiology Hydrogen-Ion Concentration Kinetics Magnesium/pharmacology Models, Chemical Phosphates/metabolism Saccharomyces cerevisiae/drug effects,metabolism,physiology Sodium/metabolism

Chemicals

Phosphates Sodium Magnesium Calcium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Roomans G M

Borst-Pauwels G W

References (12)

12 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1780521

Published

1979-03-15

Pages

521-7

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1186550

Subset

External Links

PubMed source DOI: 10.1042/bj1780521 PMC Full Text View journal details

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Interaction of cations with phosphate uptake by Saccharomyces cerevisiae. Effects of surface potential.

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