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PMID: 6306562 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Methylation of the T-DNA in Agrobacterium tumefaciens and in several crown gall tumors.

Nucleic acids research ·Vol. 11 ·No. 1 ·1983-01-11 ·Pages 159-74

Gelvin SB, Karcher SJ, DiRita VJ

Abstract

Methylation of the T-DNA in Agrobacterium tumefaciens and in four octopine-type (A6S/2, E9, 15955/1, 15955/01) and one nopaline-type (HT37#15) crown gall tumors was investigated using the isoschizomeric restriction endonucleases Msp I and Hpa II. T-DNA in the octopine-type Ti-plasmid pTiB6(806) was not methylated at the sequence 5'CCGG3' in Agrobacterium. With two possible exceptions, neither was the T-DNA of the nopaline-type Ti-plasmid pTiT37 methylated in the bacterium. In all tumor lines investigated, at least one copy of the T-DNA was not methylated. DNA methylation was not detected in the lines A6S/2, 15955/1, HT37#15, and the TL region of E9. DNA methylation of some copies of TR in the E9 tumor line, and possibly in the 15955/01 line, was detected. The methylation of some copies of TR in the E9 line may indicate that not all copies of TR are transcribed in this tumor.

MeSH Terms
Cell Line DNA/genetics DNA Restriction Enzymes Methylation Plant Tumors Plasmids Rhizobium/genetics Species Specificity
Chemicals
DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gelvin S B
Karcher S J
DiRita V J
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.1.159
Published
1983-01-11
Pages
159-74
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325696
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.1.159 PMC Full Text View journal details
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