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PMID: 212732 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA modifying enzymes of Agrobacterium tumefaciens: effect of DNA topoisomerase, restriction endonuclease, and unique DNA endonuclease on plasmid and plant DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 9 ·1978-09-00 ·Pages 4097-101

LeBon JM, Kado CI, Rosenthal LJ, Chirikjian JG

Abstract

Extracts from Agrobacterium tumefaciens strain ID135 contain three enzymes that have been characterized and partially purified. The first enzyme, a DNA topoisomerase, appeared to relax only negatively twisted DNA. The second enzyme, Atu I, a type II restriction endonuclease, generated the identical DNA digestion pattern as EcoRII when several DNAs were used. The third enzyme, endonuclease A, showed a preference for superhelical DNAs as substrates. When plasmid pCK135DNA, obtained from the virulent strain IDI135 of A. tumefaciens, or plant DNA was exposed to the three enzymes, changes in DNA patterns were observed due to either conformational changes or digestion of the DNAs. These enzymes may function in vivo in the processing and incorporation of bacterial DNA in plant cells.

MeSH Terms

Animals DNA/metabolism DNA Restriction Enzymes/metabolism DNA Topoisomerases, Type I/metabolism DNA, Superhelical/metabolism Electrophoresis, Polyacrylamide Gel Endonucleases/metabolism Plants Plasmids Rhizobium/enzymology Substrate Specificity

Chemicals

DNA, Superhelical DNA Endonucleases DNA Restriction Enzymes DNA Topoisomerases, Type I

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

LeBon J M

Kado C I

Rosenthal L J

Chirikjian J G

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25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.9.4097

Published

1978-09-00

Pages

4097-101

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC336058

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.9.4097 PMC Full Text View journal details

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DNA modifying enzymes of Agrobacterium tumefaciens: effect of DNA topoisomerase, restriction endonuclease, and unique DNA endonuclease on plasmid and plant DNA.

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