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PMID: 6292833 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure-function relationship of Rous sarcoma virus leader RNA.

Nucleic acids research ·Vol. 10 ·No. 17 ·1982-09-11 ·Pages 5183-96

Darlix JL, Zuker M, Spahr PF

Abstract

Cells infected by RSV synthesize viral 35S RNA as well as subgenomic 28S and 22S RNAs coding for the Env and Src genes respectively. In addition, at least the 5' 101 nucleotides of the leader are also conserved and we have shown previously that this sequence contains a strong ribosome binding site (J.-L. Darlix et al., J. Virol. 29, 597). We now report the RNA sequence of Rous Sarcoma virus (RSV) leader RNA and propose a folding of this 5' untranslated region which brings the Cap, the initiation codon for Gag and the strong ribosome binding site close to each other. We also show that ribosomes protect a sequence just upstream from initiator Aug of Gag in vitro, and believed to interact with part of the strong ribosome binding site according to the folding proposed for the leader RNA.

MeSH Terms
Animals Avian Sarcoma Viruses/genetics Base Sequence Chick Embryo Fibroblasts Genes, Viral Nucleic Acid Conformation RNA, Viral/genetics Ribonuclease T1 Ribosomes/metabolism
Chemicals
RNA, Viral Ribonuclease T1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Darlix J L
Zuker M
Spahr P F
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.17.5183
Published
1982-09-11
Pages
5183-96
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320864
Subset
IM
Databases
GENBANK
J02339
External Links
PubMed source DOI: 10.1093/nar/10.17.5183 PMC Full Text View journal details
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