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PMID: 6255413 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of the secondary and tertiary structures of Rous sarcoma virus RNA.

Nucleic acids research ·Vol. 8 ·No. 15 ·1980-08-11 ·Pages 3335-54

Darlix JL, Schwager M, Spahr PF, Bromley PA

Abstract

The secondary and tertiary structures of the 35S RNA of Rous Sarcoma virus have been investigated. T1 RNase partial digests have been first resolved into their components by gel electrophoresis under non denaturing conditions and then each component analyzed further by various techniques. More than one hundred structured fragments have thus been isolated and shown to consist of several individual nucleotide sequences located far apart on the genome. On the basis of the results, a cloverleaf model for the structure of RSV 35S RNA is proposed that has implications for the various biological functions of this RNA.

MeSH Terms
Animals Avian Sarcoma Viruses/genetics Chick Embryo Electrophoresis, Polyacrylamide Gel Macromolecular Substances Nucleic Acid Conformation RNA, Viral/metabolism Ribonuclease T1
Chemicals
Macromolecular Substances RNA, Viral Ribonuclease T1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Darlix J L
Schwager M
Spahr P F
Bromley P A
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.15.3335
Published
1980-08-11
Pages
3335-54
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324156
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.15.3335 PMC Full Text View journal details
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