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PMID: 219249 Published · ppublish English Journal Article

In vitro, the major ribosome binding site on Rous sarcoma virus RNA does not contain the nucleotide sequence coding for the N-terminal amino acids of the gag gene product.

Journal of virology ·Vol. 29 ·No. 2 ·1979-02-00 ·Pages 597-611

Darlix JL, Spahr PF, Bromley PA, Jaton JC

Abstract

Ribosomes from the reticulocyte lysate bind strongly and mainly to a region located in the 5' end of the Rous sarcoma virus RNA molecule between residues 9 and 53. This binding involves the participation of initiator tRNA and is sensitive to inhibitors of initiation of protein synthesis such as 7-methyl-GMP and aurintricarboxylic acid. The nucleotide sequence of this ribosome binding site has been determined: it conatains a GUG codon centered at position 26 that is not in phase with any termination codon within the 5' end nucleotide sequence of the RNA that we have analyzed (101 residues). However, the predicted N-terminal amino acid sequence starting from this GUG codon (or even from any AUG or GUG codon in the 5' end of the RNA) does not coincide with that of the in vitro-synthesized product of the 5' end proximal gag gene. Nevertheless, inhibition of ribosome binding to this site is accompanied by an inhibition of the in vitro translation of the gag gene.

MeSH Terms
Amino Acid Sequence Avian Sarcoma Viruses/analysis,metabolism Base Sequence Binding Sites/drug effects Guanosine Monophosphate/analogs & derivatives,pharmacology Nucleotides/analysis RNA, Viral/analysis,metabolism Reticulocytes Ribosomes/metabolism
Chemicals
Nucleotides RNA, Viral Guanosine Monophosphate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Darlix J L
Spahr P F
Bromley P A
Jaton J C
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34 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.29.2.597-611.1979
Published
1979-02-00
Pages
597-611
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353193
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.29.2.597-611.1979 PMC Full Text View journal details
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