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PMID: 6281464 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The leader sequence of the subgenomic mRNA's of Rous sarcoma virus is approximately 390 nucleotides.

Journal of virology ·Vol. 41 ·No. 2 ·1982-02-00 ·Pages 527-34

Hackett PB, Swanstrom R, Varmus HE, Bishop JM

Abstract

The subgenomic mRNA's of Rous sarcoma virus share a common 5' leader sequence spliced from genomic RNA. We have examined the 5' terminal sequences of four Rous sarcoma virus RNAs: virion RNA and three species of intracellular mRNA which direct the synthesis of the RSV gene products. The lengths of the leaders on the RNAs were determined by the extent that they could protect cloned Rous sarcoma virus DNA fragments from S1 nuclease digestion after RNA-DNA hybridization. We found that the subgenomic mRNA's that direct the synthesis of the env and src gene products have uninterrupted spliced leader sequences of approximately 390 nucleotides, whereas virion RNA and full-length intracellular viral RNA have 5' termini homologous to the cloned viral DNA probe over at least the first 735 bases. In the accompanying manuscript we have determined the nucleotide sequence of the 5' end of the Rous sarcoma virus genome, including the candidate splice donor site identified here (Swanstrom et al., J. Virol. 41:535-541, 1982).

MeSH Terms
Avian Sarcoma Viruses/genetics Base Sequence Genes Molecular Weight RNA Splicing RNA, Messenger/genetics RNA, Viral/genetics Viral Envelope Proteins Viral Proteins/genetics
Chemicals
RNA, Messenger RNA, Viral Viral Envelope Proteins Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hackett P B
Swanstrom R
Varmus H E
Bishop J M
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46 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.41.2.527-534.1982
Published
1982-02-00
Pages
527-34
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256781
Subset
IM
Grants
NCI NIH HHS · 1T32 CA 09043 · United States
NCI NIH HHS · CA 12705 · United States
NCI NIH HHS · CA 19287 · United States
External Links
PubMed source DOI: 10.1128/JVI.41.2.527-534.1982 PMC Full Text View journal details
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