Home LiteratureArticle Details
PMID: 6245059 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Assembly of the mitochondrial membrane system: isolation of mitochondrial transfer ribonucleic acid mutants and characterization of transfer ribonucleic acid genes of Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 141 ·No. 3 ·1980-03-00 ·Pages 1086-97

Berlani RE, Pentella C, Macino G, Tzagoloff A

Abstract

A method is described for isolating cytoplasmic mutants of Saccharomyces cerevisiae with lesions in mitochondrial transfer ribonucleic acids (tRNA's). The mutants were selected for slow growth on glycerol and for restoration of wild-type growth by cytoplasmic "petite" testers that contain regions of mitochondrial deoxyribonucleic acid (DNA) with tRNA genes. The aminoacylated mitochondrial tRNA's of several presumptive tRNA mutants were analyzed by reverse-phase chromatography on RPC-5. Two mutant strains, G76-26 and G76-35, were determined to carry mutations in the cysteine and histidine tRNA genes, respectively. The cysteine tRNA mutant was used to isolate cytoplasmic petite mutants whose retained segments of mitochondrial DNA contain the cysteine tRNA gene. The segment of one such mutant (DS504) was sequenced and shown to have the cysteine, histidine, and threonine tRNA genes. The structures of the three mitochondrial tRNA's were deduced from the DNA sequence.

MeSH Terms
Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Fungal/genetics DNA, Mitochondrial/genetics Genes Mitochondria Mutation Nucleic Acid Conformation RNA, Fungal/genetics RNA, Transfer/genetics Recombination, Genetic Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal DNA, Mitochondrial RNA, Fungal RNA, Transfer DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Berlani R E
Pentella C
Macino G
Tzagoloff A
References (37)
37 references, click to expand
  1. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
    Biochemistry. 1968 Feb;7(2):668-74 PMID: 4868544
  2. Hybridization of mitochondrial transfer RNA's with mitochondrial and nuclear DNA of grande (wild type) yeast.
    J Mol Biol. 1972 Feb 14;63(3):431-40 PMID: 4552406
  3. Histidine regulation in Salmonella typhimurium. 13. Nucleotide sequence of histidine transfer ribonucleic acid.
    J Biol Chem. 1972 May 25;247(10):2989-3000 PMID: 4337503
  4. Studies on energy-linked reactions. Isolation and characterisation of oligomycin-resistant mutants of Saccharomyces cerevisiae.
    Eur J Biochem. 1973 Jan 15;32(2):301-11 PMID: 4569077
  5. Mitochondrial genetics. VI. The petite mutation in Saccharomyces cerevisiae: interrelations between the loss of the p+ factor and the loss of the drug resistance mitochondrial genetic markers.
    Genetics. 1974 Feb;76(2):195-219 PMID: 4595642
  6. Physical and genetic organization of petite and grande yeast mitochondrial DNA. IV. In vivo transcription products of mitochondrial DNA and localization of 23 S ribosomal RNA in petite mutants of saccharomyces cerevisiae.
    J Mol Biol. 1974 Sep 5;88(1):185-203 PMID: 4613841
  7. Assembly of the mitochondrial membrane system: isolation of nuclear and cytoplasmic mutants of Saccharomyces cerevisiae with specific defects in mitochondrial functions.
    J Bacteriol. 1975 Jun;122(3):826-31 PMID: 168180
  8. Assembly of the mitochondrial membrane system. Cytoplasmic mutants of Saccharomyces cerevisiae with lesions in enzymes of the respiratory chain and in the mitochondrial ATPase.
    J Biol Chem. 1975 Oct 25;250(20):8236-42 PMID: 171256
  9. Heterogeneity of mitochondrial DNA from Saccharomyces cerevisiae and genetic information for tRNA.
    Nucleic Acids Res. 1975 Oct;2(10):1777-86 PMID: 1103084
  10. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli.
    J Mol Biol. 1975 Nov 5;98(3):551-64 PMID: 1104875
  11. Localization in yeast mitochondrial DNA of mutations expressed in a deficiency of cytochrome oxidase and/or coenzyme QH2-cytochrome c reductase.
    Eur J Biochem. 1976 Jan 2;61(1):27-41 PMID: 173553
  12. The organization of genes in yeast mitochondrial DNA II. The physical map of EcoRI and HindII + III fragments.
    Mol Gen Genet. 1975 Dec 30;143(1):53-64 PMID: 765743
  13. Assembly of the mitochondrial membrane system XVI. Modified form of the ATPase proteolipid in oligomycin-resistant mutants of Saccharomyces cerevisiae.
    FEBS Lett. 1976 Jun 15;65(3):391-5 PMID: 133820
  14. A specific endonuclease from Haemophilus haemolyticus.
    J Mol Biol. 1976 May 5;103(1):199-208 PMID: 1085372
  15. Assembly of the mitochondrial membrane system. XVIII. Genetic loci on mitochondrial DNA involved in cytochrome b biosynthesis.
    Mol Gen Genet. 1976 Nov 24;149(1):33-42 PMID: 796670
  16. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  17. Nucleotide sequence of bacteriophage phi X174 DNA.
    Nature. 1977 Feb 24;265(5596):687-95 PMID: 870828
  18. Yeast mitochondrial DNA specifies tRNA for 19 amino acids. Deletion mapping of the tRNA genes.
    Biochemistry. 1977 Oct 18;16(21):4672-7 PMID: 334246
  19. Fine structure physical mapping of 4S RNA genes on mitochondrial DNA of Saccharomyces cerevisiae.
    Mol Gen Genet. 1977 Sep 9;154(3):255-62 PMID: 337105
  20. Study of yeast mitochondrial tRNAs by two-dimensional polyacrylamide gel electrophoresis: characterization of isoaccepting species and search for imported cytoplasmic tRNAs.
    Nucleic Acids Res. 1977 Oct;4(10):3497-510 PMID: 337238
  21. The mapping of mutations in tRNA and cytochrome oxidase genes located in the cap-par segment of the mitochondrial genome of S. cerevisiae.
    Mol Gen Genet. 1977 Dec 14;158(1):35-45 PMID: 203841
  22. Mitochondrial transfer RNAs in yeast: identification of isoaccepting transfer RNAs.
    Biochemistry. 1978 May 2;17(9):1628-34 PMID: 350272
  23. Sequence homologies of (guanosine + cytidine)-rich regions of mitochondrial DNA of Saccharomyces cerevisiae.
    J Biol Chem. 1979 Jan 10;254(1):42-3 PMID: 363718
  24. Assembly of the mitochondrial membrane system: mutations in the pho2 locus of the mitochondrial genome of Saccharomyces cerevisiae.
    Eur J Biochem. 1978 Dec 1;92(1):279-87 PMID: 153229
  25. Site specific endonuclease from Fusobacterium nucleatum.
    Nucleic Acids Res. 1979 Jan;6(1):1-15 PMID: 424284
  26. Assembly of the mitochondrial membrane system. The DNA sequence of a mitochondrial ATPase gene in Saccharomyces cerevisiae.
    J Biol Chem. 1979 Jun 10;254(11):4617-23 PMID: 155696
  27. Assembly of the mitochondrial membrane system: two separate genes coding for threonyl-tRNA in the mitochondrial DNA of Saccharomyces cerevisiae.
    Mol Gen Genet. 1979 Jan 31;169(2):183-8 PMID: 375006
  28. Physical mapping of the yeast mitochondrial genome: derivation of the fine structure and gene map of strain D273-10B and comparison with a strain (MH41-7B) differing in genome size.
    Mol Gen Genet. 1979 Feb 16;170(1):25-48 PMID: 375015
  29. Nucleotide sequence of the mitochondrial structural gene for subunit 9 of yeast ATPase complex.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1663-7 PMID: 156363
  30. The isolation of mitochondrial and nuclear mutants of Saccharomyces cerevisiae with specific defects in mitochondrial functions.
    Methods Enzymol. 1979;56:95-106 PMID: 223014
  31. The genetic map of transfer RNA genes of yeast mitochondria: correction and extension.
    Mol Gen Genet. 1979 Mar 5;170(3):261-75 PMID: 379543
  32. Assembly of the mitochondrial membrane system. DNA sequence of subunit 2 of yeast cytochrome oxidase.
    J Biol Chem. 1979 Sep 25;254(18):9324-30 PMID: 225327
  33. The nucleotide sequence of the mitochondrial genome of a spontaneous "petite" mutant of yeast.
    Mol Gen Genet. 1979 Jul 24;174(3):335-7 PMID: 384171
  34. Nucleotide sequence of the mitochondrial structural genes for cysteine-tRNA and histidine-tRNA of yeast.
    Nucleic Acids Res. 1979 Jul 25;6(10):3255-66 PMID: 384365
  35. Use of the UGA terminator as a tryptophan codon in yeast mitochondria.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3784-5 PMID: 226981
  36. Sequence analysis of two yeast mitochondrial DNA fragments containing the genes for tRNA Ser UCR and tRNA Phe UUY.
    J Biol Chem. 1979 Nov 25;254(22):11735-40 PMID: 387778
  37. Assembly of the mitochondrial membrane system: sequences of yeast mitochondrial valine and an unusual threonine tRNA gene.
    Cell. 1979 Sep;18(1):47-53 PMID: 389433
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.141.3.1086-1097.1980
Published
1980-03-00
Pages
1086-97
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC293786
Subset
IM
Databases
GENBANK
K00537, V00697
External Links
PubMed source DOI: 10.1128/jb.141.3.1086-1097.1980 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2