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PMID: 375006 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Assembly of the mitochondrial membrane system: two separate genes coding for threonyl-tRNA in the mitochondrial DNA of Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 169 ·No. 2 ·1979-01-31 ·Pages 183-8

Macino G, Tzagoloff A

Abstract

1. Mitochondria of Saccharomyces cerevisiae contain two tRNA's that are acylated with threonine. The two isoaccepting species (tRNA1Thr and tRNA2Thr) can be separated by reversed-phase chromatography on RPC-5. 2. A cytoplasmic mutant has been isolated which lacks tRNA1Thr but has normal levels of tRNA2Thr. This mutation was previously shown to map between the oxi 1 and oxi 2 loci on mitochondrial DNA. 3. tRNA1Thr and tRNA2Thr hybridize to wild type mitochondrial but not nuclear DNA and are capable of partially competing with each other. Hybridization of each species to different segments of mitochondrial DNA isolated from p- clones indicate that there are two threonyl tRNA genes. One gene is located between oxi 1 and oxi 2 and codes for tRNA1Thr. The second gene codes for tRNA2Thr and is near the cap locus. 4. Binding assays to E. coli ribosomes indicate that tRNA2Thr recognizes the threonine triplet ACA and may also recognize the other three triplets but with a much lower efficiency. None of the four codons for threonine stimulate the binding of tRNA1Thr to the ribosomes.

MeSH Terms

Codon DNA, Mitochondrial/genetics Genes Membranes/ultrastructure Morphogenesis Mutation Nucleic Acid Hybridization RNA, Transfer/genetics Saccharomyces cerevisiae/genetics Threonine/genetics

Chemicals

Codon DNA, Mitochondrial Threonine RNA, Transfer

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Macino G

Tzagoloff A

References (14)

14 references, click to expand

Physical and genetic organization of petite and grande yeast mitochondrial DNA. IV. In vivo transcription products of mitochondrial DNA and localization of 23 S ribosomal RNA in petite mutants of saccharomyces cerevisiae.
J Mol Biol. 1974 Sep 5;88(1):185-203 PMID: 4613841
The isolation of mitochondrial and nuclear mutants of Saccharomyces cerevisiae with specific defects in mitochondrial functions.
Methods Enzymol. 1979;56:95-106 PMID: 223014
Large-scale preparation of yeast "soluble" ribonucleic acid.
Biochem Biophys Res Commun. 1963 Jan 31;10:186-8 PMID: 13961341
Deletion mapping of mitochondrial transfer RNA genes in Saccharomyces cerevisiae by means of cytoplasmic petite mutants.
Mol Gen Genet. 1976 Apr 23;145(1):7-17 PMID: 775312
Heterogeneity of mitochondrial DNA from Saccharomyces cerevisiae and genetic information for tRNA.
Nucleic Acids Res. 1975 Oct;2(10):1777-86 PMID: 1103084
The mapping of mutations in tRNA and cytochrome oxidase genes located in the cap-par segment of the mitochondrial genome of S. cerevisiae.
Mol Gen Genet. 1977 Dec 14;158(1):35-45 PMID: 203841
Yeast mitochondrial DNA specifies tRNA for 19 amino acids. Deletion mapping of the tRNA genes.
Biochemistry. 1977 Oct 18;16(21):4672-7 PMID: 334246
Hybridization of mitochondrial transfer RNA's with mitochondrial and nuclear DNA of grande (wild type) yeast.
J Mol Biol. 1972 Feb 14;63(3):431-40 PMID: 4552406
Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.
Biochim Biophys Acta. 1971 Feb 11;228(3):770-4 PMID: 5572614
Coding specificity of Escherichia coli leucine transfer ribonucleic acids and effect of bacteriophage T2 infection.
J Mol Biol. 1970 Sep 14;52(2):179-93 PMID: 4320938
Mitochondrial DNA's from respiratory-sufficient and cytoplasmic respiratory-deficient mutant yeast.
J Mol Biol. 1970 Feb 28;48(1):23-42 PMID: 5448588
Selective translation of T4 template RNA by ribosomes from T4-infected Escherichia coli.
Proc Natl Acad Sci U S A. 1969 Sep;64(1):345-51 PMID: 4904642
[Comparative study of cytoplasmic and mitochondrial valyl tRNA of yeast].
Biochimie. 1973;55(3):291-6 PMID: 4583049
Native and imported transfer RNA in mitochondria.
J Mol Biol. 1975 Nov 25;99(1):37-50 PMID: 813002

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00271669

Published

1979-01-31

Pages

183-8

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00271669

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Assembly of the mitochondrial membrane system: two separate genes coding for threonyl-tRNA in the mitochondrial DNA of Saccharomyces cerevisiae.

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