Heterogeneity of mitochondrial DNA from Saccharomyces cerevisiae and genetic information for tRNA.

Nucleic acids research ·Vol. 2 ·No. 10 ·1975-10-00 ·Pages 1777-86

Baldacci G, Carnevali F, Frontali L, Leoni L, Macino G, Palleschi C

Abstract

Mitochondrial DNA from wild-type Saccharomyces cerevisiae and from an "extreme" petite mutant were analyzed by hybridization of several tRNAs on DNA fragments of different buoyant density, obtained by sonication and fractionation on a CsCl gradient. The hybridization patterns show that the genes for tRNAser, tRNAphe, tRNAhis, tRNAval, tRNAileu are present on wild-type mitochondrial DNA, while only genes for tRNAser and tRNAhis are present on petite mitochondrial DNA; moreover the hybridization patterns indicate that these genes are not clustered and suggest that more than one gene might exist for tRNAser and tRNAhis.

MeSH Terms

Centrifugation, Density Gradient DNA, Mitochondrial/analysis Kinetics Mutation Nucleic Acid Hybridization RNA, Transfer Saccharomyces cerevisiae/analysis

Chemicals

DNA, Mitochondrial RNA, Transfer

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Baldacci G

Carnevali F

Frontali L

Leoni L

Macino G

Palleschi C

References (14)

14 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/2.10.1777

Published

1975-10-00

Pages

1777-86

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343545

Subset

External Links

PubMed source DOI: 10.1093/nar/2.10.1777 PMC Full Text View journal details

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Heterogeneity of mitochondrial DNA from Saccharomyces cerevisiae and genetic information for tRNA.

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