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PMID: 765743 Published · ppublish English Journal Article

The organization of genes in yeast mitochondrial DNA II. The physical map of EcoRI and HindII + III fragments.

Molecular & general genetics : MGG ·Vol. 143 ·No. 1 ·1975-12-30 ·Pages 53-64

Sanders JP, Borst P, Weijers PJ

Abstract

1. We have isolated large fragments of the mtDNA of the yeast Saccharomyces carlsbergensis and digested these with restriction endonucleases. The digestion products were separated by electrophoresis in agarose gels. 2. Endonucleases EcoRI, HindII + III, HpaI, HindIII and HapII yield 9, 11, 6, 0 and greater than 80 fragments, respectively. 3. By analysis of partial digestion products and by redigesting the fragments obtained with one endonuclease with a second, we have established the order of all EcoRI and HindII + III fragments. The map is circular and its contour length is 22.1 +/- 0.35 mum, in good agreement with earlier estimates of the size of yeast mtDNA, using electron microscopy and renaturation kinetics. 4. A comparison of the fragmentation pattern of mtDNAs from S. carlsbergensis and various strains of Saccharomyces cerevisiae with endonuclease HindII + III suggests that the overall gene order is similar.

MeSH Terms
DNA Restriction Enzymes DNA, Circular/analysis DNA, Mitochondrial/analysis Extrachromosomal Inheritance Genes Molecular Weight Nucleic Acid Hybridization Saccharomyces/analysis Saccharomyces cerevisiae/analysis Species Specificity
Chemicals
DNA, Circular DNA, Mitochondrial DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sanders J P
Borst P
Weijers P J
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41 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00269420
Published
1975-12-30
Pages
53-64
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00269420
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