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PMID: 163917 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Structure and function of herpesvirus genomes. I. comparison of five HSV-1 and two HSV-2 strains by cleavage their DNA with eco R I restriction endonuclease.

Journal of virology ·Vol. 15 ·No. 4 ·1975-04-00 ·Pages 726-32

Skare J, Summers WP, Summers WC

Abstract

The restriction endonuclease Eco R I cleaves HSV-1 and HSV-2 DNA into specific fragments that can be resolved by agarose gel electrophoresis. Comparison of HSV-1 strains KOS, 14-012, MP, F, and CI 101, and HSV-2 strains 333 and 186, suggests that the DNAs from type 1 strains are similar but not identical, and that the type 2 strains differ greatly from type 1 strains. The molecular lengths of the fragments have been determined by electron microscopy and can be used to calibrate gel electrophoretic analyses of DNA fragments.

MeSH Terms
Agar Autoradiography DNA, Viral Electrophoresis Endonucleases Genes Genetics, Microbial Herpesvirus 1, Bovine Microscopy, Electron Molecular Weight Simplexvirus
Chemicals
DNA, Viral Agar Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Skare J
Summers W P
Summers W C
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25 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.15.4.726-732.1975
Published
1975-04-00
Pages
726-32
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354515
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.15.4.726-732.1975 PMC Full Text View journal details
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