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PMID: 6171557 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Enhancement of ribosomal ribonucleic acid synthesis by deoxyribonucleic acid gyrase activity in Escherichia coli.

Journal of bacteriology ·Vol. 148 ·No. 3 ·1981-12-00 ·Pages 782-7

Oostra BA, van Vliet AJ, Ab G, Gruber M

Abstract

The effect of the deoxyribonucleic acid (DNA) gyrase inhibitors coumermycin A1, novobiocin, and oxolinic acid on ribonucleic acid (RNA) synthesis in Escherichia coli was studied in vivo and in vitro. Preferential inhibition of ribosomal RNA (rRNA) synthesis was observed. No effect of oxolinic acid and coumermycin on rRNA synthesis was seen in mutants having a DNA gyrase which is resistant to these inhibitors. In a temperature-sensitive DNA gyrase mutant rRNA synthesis was decreased at nonpermissive temperatures. Thus, a functional DNA gyrase is required for rRNA synthesis. Purified DNA gyrase had no effect on rRNA synthesis in a purified system. However, DNA gyrase does show preferential stimulation of rRNA synthesis in a system supplemented with other proteins. Apparently, DNA gyrase stimulation of rRNA synthesis requires another protein.

MeSH Terms
Aminocoumarins Bacterial Proteins/biosynthesis Coumarins/pharmacology DNA Topoisomerases, Type II/metabolism Escherichia coli/metabolism Novobiocin/pharmacology Oxolinic Acid/pharmacology RNA, Bacterial/biosynthesis RNA, Ribosomal/biosynthesis
Chemicals
Aminocoumarins Bacterial Proteins Coumarins RNA, Bacterial RNA, Ribosomal Novobiocin DNA Topoisomerases, Type II Oxolinic Acid coumermycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Oostra B A
van Vliet A J
Ab G
Gruber M
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.148.3.782-787.1981
Published
1981-12-00
Pages
782-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216275
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.148.3.782-787.1981 PMC Full Text View journal details
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