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PMID: 6253912 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Involvement of DNA gyrase in the transcription of ribosomal RNA.

Nucleic acids research ·Vol. 8 ·No. 18 ·1980-09-25 ·Pages 4235-46

Oostra BA, Gruber M

Abstract

The DNA gyrase inhibitor novobiocin specifically inhibits the transcription of ribosomal RNA in vivo while protein synthesis and the mRNA transcription are only partly affected. In vitro the novobiocin inhibition is only observed when protein fraction, which stimulates ribosomal RNA synthesis, is present. These results indicate that DNA gyrase is involved in the transcription of ribosomal RNA, probably at an initiation step.

MeSH Terms
Bacteriophage lambda/metabolism DNA Topoisomerases, Type II/metabolism Escherichia coli/metabolism Novobiocin/pharmacology Protein Biosynthesis RNA, Messenger/biosynthesis RNA, Ribosomal/biosynthesis Transcription, Genetic/drug effects
Chemicals
RNA, Messenger RNA, Ribosomal Novobiocin DNA Topoisomerases, Type II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Oostra B A
Gruber M
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29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.18.4235
Published
1980-09-25
Pages
4235-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324231
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.18.4235 PMC Full Text View journal details
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